This is a knockout-validated antibody summary, based on the publication "BRI2 inhibits amyloid beta-peptide precursor protein processing by interfering with the docking of secretases to the substrate", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1

Company: Millipore

Antibody: APP

Catalog number: MAB348

Summary: Mouse monoclonal IgG1 against an epitope mapping amino acids 66-81 of the N-terminus Alzheimer precursor A4 (pre A4695) fusion protein. The antibody recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa. Reacts with canine, human, mouse, monkey, pig, rat and fish. Recommended for western blot, immunofluorescence, and immunohistochemistry.

The antibody is known to cross react with APLP2.

Western blot

Brain membrane extracts from WT, Bri2−/− and APP−/− mice. Mouse brains were homogenized in 1 ml of HEPES–sucrose buffer (20 mM HEPES/NaOH, pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.25 M sucrose) supplemented with protease inhibitors.

Please see Figure 2b in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.