This is a knockout-validated antibody summary, based on the publications "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity", as cited below [1] and "Inhibition of Lung Tumor Development in ApoE Knockout Mice via Enhancement of TREM-1 Dependent NK Cell Cytotoxicity" for western blot (figure 3d) and immunohistochemistry (figure 1c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis (A) and immunofluorescence staining (B) of brains from WT and Apoe-deficient mice show total depletion of the protein. Scale bars = 50μm, Blue lectin positive area indicate microvessels; green GFAP positive area indicate astrocytes; red APOE positive area indicate APOE; n = 5 each group. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: APOE

Catalog number: ab1907

Summary: Mouse monoclonal IgG1 against a synthetic peptide spanning the polymorphic amino acid position 158 of ApoE. Reacts with mouse and human. Suitable for western blot, immunoprecipitation, immunohistochemistry (frozen and paraffin), immunocytochemistry/immunofluorescence and ELISA.

Western blot | Immunohistochemistry

Brains from WT and ApoE-knockout mice. WB: tissues were lysed in RIPA Lysis Buffer with a protease inhibitor cocktail (05892791001; Roche, Basel, Switzerland) and homogenized at 4°C until lysis was complete. IHC: mice were perfused with 4% paraformaldehyde (PFA), the whole brains were harvested and coronary frozen sections were made.

WB: 5% bovine serum albumin at room temperature for 1 h.

WB: At 4°C overnight.

WB: for 1 h.

IHC: DyLight 594 AffiniPure Goat Anti-Mouse IgG (A23410, Abbkine).

WB: ECL Western Blotting kit (32209; Thermo Scientific, Waltham, MA) and chemiluminescence imaging system (ChemiDoc XRS+; Bio-Rad, Hercules, CA).

The same clone (E6D7) is sold as BioLegend 803403, 803401, 803404, 803405; Abcam ab1907; Invitrogen MA1-91063; MilliporeSigma A8599.