APG5L/ATG5 antibody | knockout validation | Abcam ab108327

This is a knockout-validated antibody summary, based on the publications "Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery", as cited below [1], "TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress" for western blot knockout validation (figure 1e) [2] and "TMEM41B Is a Pan-flavivirus Host Factor" for western blot knockout validation (figure s2a) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: APG5L/ATG5

Catalog number: ab108327

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human APG5L/ATG5. Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), and immunocytochemistry / immunofluorescence.

Validation Method

Western blot

Sample

Atg5wt, Atg5−/−, and Atg5+/+ MEFs. protein lysates were prepared in Giordano lysis buffer.

Figure

Atg5wt, Atg5−/−, and Atg5+/+ MEFs were infected with R124 or jin-3 at an MOI of 1.Lysates were analyzed for the Atg5, and actin. From [1].

References
  1. Kemp V, Dautzenberg I, Limpens R, van den Wollenberg D, Hoeben R. Oncolytic Reovirus Infection Is Facilitated by the Autophagic Machinery. Viruses. 2017;9: pubmed publisher
  2. Guo S, Chen Y, Yang Y, Zhang X, Ma L, Xue X, et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis. 2021;12:42 pubmed publisher
  3. Hoffmann H, Schneider W, Rozen Gagnon K, Miles L, Schuster F, Razooky B, et al. TMEM41B Is a Pan-flavivirus Host Factor. Cell. 2021;184:133-148.e20 pubmed publisher