This is a knockout-validated antibody summary, based on the publication "Sequential cancer mutations in cultured human intestinal stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1κ

Company: Calbiochem

Antibody: APC

Catalog number: MABC202

Clone: FE9. This clone is also sold as Invitrogen MA1-91039.

Summary: Mouse monoclonal IgG1κ against a linear peptide corresponding to the N-terminus of human APC. Recommended for the detection of APC of human origin by western blot.

Western blot

Wildtype and APCKO human organoids. Samples were lysed using RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% SDS, 0.5% Na-Deoxycholate, 1% NP-40) containing Complete protease inhibitors (Roche).

Please see Figure 2f in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.