AP2M1 antibody | knockout validation | Abcam ab75995

This is a knockout-validated antibody summary, based on the publication "Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

AP2M1 antibody | knockout validation | Abcam ab75995 figure 1
Figure 1. AP2M1−/− and WT 293T cells were treated with CHX before virus infection (10 MOI). Nuclear (Nuc) and cytoplasmic (Cyto) fractions were separated and detected at 2 hpi by Western blotting. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: AP2M1

Catalog number: ab75995

Summary: Rabbit monoclonal antibody against a synthetic peptide within human AP2M1 (aa 350 to the C-terminus). Reacts with mouse, rat and human. Suitable for western blot.

Validation Method

Western blot

Sample

WT and AP2M1−/− 293T cells.

References
  1. Yuan S, Chu H, Huang J, Zhao X, Ye Z, Lai P, et al. Viruses harness YxxØ motif to interact with host AP2M1 for replication: A vulnerable broad-spectrum antiviral target. Sci Adv. 2020;6:eaba7910 pubmed publisher