This is a knockout-validated antibody summary, based on the publication "TWEAK/Fn14 signaling is required for liver regeneration after partial hepatectomy in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 1 in [1]. Western blot of lysates of liver from wild-type and Fn14 knockout mice undergoing liver regeneration following partial hepatectomy were probed with anti-Fn14 antibody. Actin serves as a loading control.

Mouse monoclonal

Company: BioLegend

Antibody: Fn14

Catalog number: 314102

Summary: Mouse monoclonal antibody raised against human Fn14. Supplier recommended for WB, IF, IHC and flow cytometry. Reacts with human and mouse Fn14.

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Figure 2. From Figure 1 in [1]. Liver slices from wild-type and Fn14 knockout mice undergoing regeneration following partial hepatectomy. Fn14 detected with anti-Fn14 antibody.

Western blot

Immunohistochemistry

Western blot: Lysates of livers from wild-type and Fn14 knockout mice undergoing regeneration following partial hepatectomy.

Immunohistochemistry: formalin-fixed liver samples.

Western blot: not stated

Immunohistochemistry: Dako Protein Block (Dako).

Western blot: anti-Fn14 antibody.

Immunohistochemistry: antiFn14 antibody.

The same clone (ITEM-4) is sold as Bio-Rad MCA4699, MCA4699A647, MCA4699FT, MCA4699F, MCA4699GA, MCA4699A488, MCA4699PE; BioLegend 314102, 314103, 314106; Santa Cruz Biotechnology sc-56250; LifeSpan Biosciences LS-C105966, LS-C106715, LS-C106982, LS-C187520, LS-C188541, LS-C188542; Abnova MAB11065; Abcam ab21127.