ANT2/SLC25A5 antibody | knockout validation | Cell Signaling 14671

This is a knockout-validated antibody summary, based on the publication "Inhibition of Mitochondrial Permeability Transition by Deletion of the ANT Family and CypD", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ANT2/SLC25A5 antibody | knockout validation | Cell Signaling 14671 figure 1
Figure 1. Western blots for ANT2 and ANT4 from isolated liver mitochondria from the indicated gene-targeted mice showing that ANT4 is induced for the first time in the absence of the Ant1 and Ant2 genes. A loading control for ComV is shown. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: ANT2/SLC25A5

Catalog number: EEEEE

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly156 of human ANT2/SLC25A5 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and ANT1/ANT2-KO liver mitochondria lysates. Mitochondria were resuspended in radioimmunoprecipitation buffer containing protease inhibitor cocktails (Roche).

Primary incubation

1:500 dilution.

References
  1. Karch J, Bround M, Khalil H, Sargent M, Latchman N, Terada N, et al. Inhibition of mitochondrial permeability transition by deletion of the ANT family and CypD. Sci Adv. 2019;5:eaaw4597 pubmed publisher