This is a knockout-validated antibody summary, based on the publication "ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting showing ANP32B expression in eHAP1 control, ANP32B-KO and double ANP32A/B knockout (dKO) cells. From [1].

Mouse monoclonal IgG2b

Company: Proteintech

Antibody: ANP32B

Catalog number: 66160-1-Ig

Summary: Mouse monoclonal IgG2b against ANP32B fusion protein. Reacts with human by western blot, immunofluorescence and ELISA.

Western blot

Control, ANP32B-KO and double ANP32A/B knockout (dKO) eHAP1 cells. Cells were lysed in buffer containing 50 mM Tris-HCl (pH 7.8; Sigma-Aldrich), 100 mM NaCl, 50 mM KCl, and 0.5% Triton X-100 (Sigma-Aldrich), supplemented with a cOmplete EDTA-free protease inhibitor cocktail tablet (Roche).

1/1,000 dilution.

1/5,000 dilution goat α-mouse HRP (STAR117P; AbD Serotec).

Protein bands were visualized by chemiluminescence (ECL Prime Western blotting detection reagent; GE Healthcare) using a Fusion-FX imaging system (Vilber Lourmat).