AMPKα antibody | knockout validation | Cell Signaling 2793

This is a knockout-validated antibody summary, based on the publication "Elevating acetyl-CoA levels reduces aspects of brain aging", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

AMPKα antibody | knockout validation | Cell Signaling 2793 figure 1
Figure 1. Phosphorylation of AMPK and ACC1 after treatment with 1 μM of each compound was assessed by Western blotting and normalized to levels of respective total protein in wildtype (Wt) and AMPK K.O. fibroblasts (n = 3–4/group). From [1].
Antibody information

Mouse monoclonal IgG2b

Company: Cell Signaling

Antibody: AMPKα

Catalog number: 2793

Summary: Mouse monoclonal IgG2b against a purified recombinant human AMPKα1 protein. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot.

Validation Method

Western blot

Sample

Wildtype (Wt) and AMPK-KO fibroblasts.

Primary incubation

1/1,000 dilution.

Secondary incubation

1/5,000 dilution horseradish peroxidase-conjugated goat anti-mouse (BioRad).

References
  1. Currais A, Huang L, Goldberg J, Petrascheck M, Ates G, Pinto Duarte A, et al. Elevating acetyl-CoA levels reduces aspects of brain aging. elife. 2019;8: pubmed publisher