This is a knockout-examined antibody summary, based on the publication "AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. AMPK and actin levels determined with western blot analysis in WT and AMPK−/− MEF cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: AMPKα

Catalog number: 2603S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the amino-terminal sequence of human AMPKα. Reacts with human, mouse, rat and monkey. Suitable for western blot.

Western blot

WT and AMPK−/− MEF cells. Cells were washed twice with cold PBS before being scraped into ice-cold 10 mM Tris, pH 7.5, 150 mM NaCl, 5 mM EDTA 0.1% Triton X-100 and a mixture of protease and phosphatase inhibitors.

5% milk Tween-TBS for 1 h.

1:1,000 dilution in blocking solution overnight.

1:3,000 dilution peroxidase-conjugated secondary antibodies (Bio-Rad).

ECL (Biological Industries Ltd, Israel) on X-ray films (Fuji Medical).