AMPKα (Thr172) antibody | knockout validation | Cell Signaling 2535

This is a knockout-validated antibody summary, based on the publication "Elevating acetyl-CoA levels reduces aspects of brain aging", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

AMPKα (Thr172) antibody | knockout validation | Cell Signaling 2535 figure 1
Figure 1. Phosphorylation of AMPK and ACC1 after treatment with 1 μM of each compound was assessed by Western blotting and normalized to levels of respective total protein in wildtype (Wt) and AMPK K.O. fibroblasts (n = 3–4/group). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: AMPKα (Thr172)

Catalog number: 2535

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Thr172 of human AMPKα protein. Reacts with human, mouse, rat, hamster, monkey, D. melanogaster, and S. cerevisiae. It is predicted to react based on 100% sequence homology with chicken, zebrafish, bovine, and pig. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin).

Validation Method

Western blot

Sample

Wildtype (Wt) and AMPK-KO fibroblasts.

Primary incubation

1/1,000 dilution.

Secondary incubation

1/5,000 dilution horseradish peroxidase-conjugated goat anti-rabbit (BioRad).

References
  1. Currais A, Huang L, Goldberg J, Petrascheck M, Ates G, Pinto Duarte A, et al. Elevating acetyl-CoA levels reduces aspects of brain aging. elife. 2019;8: pubmed publisher