This is a knockout-validated antibody summary, based on the publication "Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Expression of wild-type or mutant AMPK in the AMPK K/O HEK 293T cell line, and effects of H2O2 boluses on wild-type and mutant AMPK-expressing cells. (A - D) AMPK K/O HEK 293T cells (UT) were transiently co-transfected with AMPK α1 (WT-FLAG (1 μg DNA) (WT) or C299A/C304A-FLAG (2 μg DNA) (MT)) and AMPK β1 (1 μg DNA) and AMPK γ1-Myc (1 μg DNA) per plate, 24 h prior to treatment and/or lysis. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: AMPKβ1/2

Catalog number: 4150

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence surrounding His233 residues of human AMPKβ1. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

The positive control is a protein transfection, not the wildtype.

AMPK KO HEK 293T cells.

Secondary antibodies were IRDye® 800 goat anti-rabbit IgG F(c) (Rockland Antibodies and Assays: number 611-132-003).

Odyssey CLx IR Imaging System with LI-COR Biosciences Image Studio Lite software.