This is a knockout-validated antibody summary, based on the publication "Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Images of brain sections prepared from WT (Upper) and KO (Lower) mice, and immunolabeled and imaged under identical conditions. Green = anti-AMIGO-1 immunolabeling. Magenta = Hoechst 33258 nuclear dye. Scale bar = 200µm. From [1].

Mouse monoclonal IgG2

Company: NeuroMab

Antibody: AMIGO-1

Catalog number: 73-317

Summary: Mouse monoclonal IgG2a against mouse

AMIGO-1 (a.a. 395-493, cytoplasmic C-terminus). Reacts with mouse, human, and rat. Suitable for immunoblot, immunocytochemistry and immunohistochemistry.

Immunohistochemistry

Brains from WT and AMIGO-1-KO mice. Following cryoprotection, all samples were frozen, and 30µm sections were generated using a freezing stage sliding microtome. Free-floating sections were processed for immunofluorescence.

10% goat serum in 0.1 M PB containing 0.3% Triton X-100 (vehicle) for 1 h.

Primary antibodies diluted in vehicle and incubated for 3 h at room temperature (RT) .

Affinity-purified species and/or mouse IgG–subclass-specific goat secondary Abs (Manning et al., 2012) conjugated to Alexa Fluors (Molecular Probes/ThermoFisher).

Low-magnification images were acquired on a Zeiss AxioObserver Z1 microscope using a 10 × /0.5 NA Fluar objective and an AxioCam HRm digital camera, and reconstructed as tiled mosaics using Axiovision 4.0 (Carl Zeiss MicroImaging, RRID:S- ciRes_000111).

The same clone (L86/33) is sold as BioLegend 827301; Neuromab 73-317, 75-317.