AIM2 antibody | knockout validation

AIM2 antibody | knockout validation | Cell Signaling 12948

This is a knockout-validated antibody summary, based on the publication "Regulatory evolution of innate immunity through co-option of endogenous retroviruses", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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AIM2 antibody | knockout validation | Cell Signaling 12948 figure 1

Figure 1. Western blot of AIM2 in wild-type and ΔMER41.AIM2 (promoter region) cells after IFNG treatment. Courtesy from the Authors [1].

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: AIM2

Catalog number: 12948

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Lys93 of human AIM2 protein. Reacts with human and it is predicted to react based on 100% sequence homology with monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Wild-type and homozygous ΔMER41.AIM2 (promoter region). Cell lysates were prepared with RIPA buffer.

Detection

SuperSignal™ West Femto Maximum Sensitivity Substrate.

References

Chuong E, Elde N, FESCHOTTE C. Regulatory evolution of innate immunity through co-option of endogenous retroviruses. Science. 2016;351:1083-7 pubmed publisher

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