This is a knockout-validated antibody summary, based on the publication "AIF-regulated oxidative phosphorylation supports lung cancer development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot analysis of AIF expression in primary pneumocytes purified from Aif + /y, Aif+/y KrasG12D, Aiffl/y, and Aiffl/y KrasG12D mice which were infected with or without Ad5-mSPC-Cre (MOI = 100) for 8 days. β-actin is shown as a loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: AIF

Catalog number: ab32516

Summary: Rabbit monoclonal IgG against a synthetic peptide within human AIF (aa 500-600, C terminal). Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation and flow cytometry.

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Figure 2. Immunohistochemical staining of AIF in lung tumors from Aiffl/y KrasG12D and Aif+/y KrasG12D mice. Note loss of AIF protein expression in the Aiffl/y KrasG12D tumor (arrows). Representative lung tumor sections are shown at 22 weeks after Ad5-CMV-Cre inhalation. Scale bar, 100 μm. From [1].

Western blot | Immunohistochemistry

Control and conditional Aif-KO mice pneumocytes and lung tumors.

1:1,000 overnight with the primary antibodies at 4 °C (diluted in 2.5% BSA in 1× TBST buffer).

WB: 1:5,000 dilution horseradish peroxidase-conjugated secondary Abs (Promega) at room temperature for 1 h.

IHC: 1:500 dilution peroxidase-conjugated anti-rabbit IgG (DAKO, P0448).

Enhanced chemiluminescence (GE Healthcare, RPN2106).