This is a knockout-validated antibody summary, based on the publication "Initial characterization of behavior and ketamine response in a mouse knockout of the post-synaptic effector gene Anks1b", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b
Company: Santa Cruz
Antibody: AIDA-1
Catalog number: sc-376610
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 847-1054 mapping near the C-terminus of human AIDA-1. Recommended for detection of AIDA-1 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine and canine.
Western blot
Cortical brain tissues from wild type, Anks1b-heterozygous and KO mice. Cortical tissue samples were lysed in Pierce® RIPA buffer (Thermo Scientific, Rockford, IL) and homogenized.
Anti-mouse IgG secondary antibody (Cell Signaling Technology, Danvers, MA).
ChemiDoc system (Bio-Rad) was used to capture and process blot images.
Western blots showing the approximately 50 and 57 kD isoforms of Anks1b (AIDA-1e and AIDA-1d, respectively) in mice of different genotypes for the tm1a and tm1b alleles. WT = wild type, KO = homozygous tm1a or tm1b, Het = heterozygote. Please see Figure 1b in the article [1].
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