AID antibody | knockout validation | eBioscience 14-5959
DOI
//dx.doi.org/10.13070/ko.en.7.1937
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

AID antibody | knockout validation | eBioscience 14-5959 figure 1
Figure 1. From Figure S2 in [1]. Western blot of lysates of B cells from AID knockout mice (vector) and AID -/- B cells retrovirally expressing human AID (+AID) probed with anti-AID antibody.
Antibody information

Rat monoclonal

IgG2a, kappa

Company: eBioscience

Antibody: AID

Catalog number: 14-5959 (Clone mAID-2)

Summary: Rat monoclonal antibody.

Supplier recommended for WB and IHC.

Reacts with human and mouse AID.

Validation Method

Western blot

Sample

Lysates of B cells from AID -/- mice and AID -/- B cells retrovirally expressing human AID.

Primary incubation

Anti-AID antibody.

References
  1. Yousif A, Stanlie A, Mondal S, Honjo T, Begum N. Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci U S A. 2014;111:E1016-24 pubmed publisher