AID antibody | knockout validation | Active Motif 39886

This is a knockout-validated antibody summary, based on the publication "Lamin B1 regulates somatic mutations and progression of B-cell malignancies", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

AID antibody | knockout validation | Active Motif 39886 figure 1
Figure 1. Western-blot analysis of AICDA expression in BL2 wt and AID-/- cells. From [1].
Antibody information

Rat monoclonal IgG2a

Company: Active Motif

Antibody: AID

Catalog number: 39886

Summary: Rat monoclonal IgG2a against a peptide corresponding to amino acids 178-198 of human AID. Reacts with human and mouse by western blot and Chromatin IP.

Validation Method

Western blot

Sample

Whole-cell lysates were prepared in NuPage LDS sample buffer (Thermo-Fisher), containing 0.1 M DTT.

Blocking agent

5% non-fat dried milk in TBS/0.05% Tween.

Secondary incubation

Horseradish peroxidase–conjugated anti-rat (DAKO/Agilent, Stockport, UK).

Detection

ECL (GE Healthcare/Amersham, Little Chalfont, UK) and ChemiDoc imaging system (BioRad, Hemel Hempstead, UK).

References
  1. Klymenko T, Bloehdorn J, Bahlo J, Robrecht S, Akylzhanova G, Cox K, et al. Lamin B1 regulates somatic mutations and progression of B-cell malignancies. Leukemia. 2018;32:364-375 pubmed publisher