This is a knockout-validated antibody summary, based on the publication "piRNA-associated proteins and retrotransposons are differentially expressed in murine testis and ovary of aryl hydrocarbon receptor deficient mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunofluorescence analysis of AhR expression in AhR+/+ and AhR-/- mouse testes at 10, 12 and 14 days postpartum (dpp). Arrowheads mark the presence of AhR (red fluorescence) in the seminiferous tubules. Testes extracted from AhR-/- mice were used as negative controls. DAPI was used to stain cell nuclei. Details of the micrographs are shown in the insets. From [1].

Mouse monoclonal IgG1

Company: Thermo Scientific

Antibody: AHR

Catalog number: MA1-514

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR. Reacts with human, mouse, and rat, and has been published to react with pig and non-human primate. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, and immunoprecipitation.

Immunohistochemistry

AhR+/+ and AhR-/- mouse testes. Testes were collected, fixed in buffered formalin and embedded in paraffin for sectioning.

1 h incubation in PBS containing 0.05% Triton X-100, 0.2% gelatin and 3% BSA.

Diluted in PBS-T/0.2% gelatin overnight at 4°C

Alexa-633 labelled secondary antibody for 1 h at room temperature.

Olympus FV1000 confocal microscope (Olympus). Objectives used were: 10× (0.40 numeric aperture) and 20× (0.70 numeric aperture). Fluorescence analysis was done using the FV10 software (Olympus).

The same clone (RPT1) is sold as Novus Biologicals NB300-530; Enzo Life Sciences ALX-804-421-R100; Abcam ab2770; Invitrogen MA1-514.