This is a knockout-validated antibody summary, based on the publication "An Argonaute phosphorylation cycle promotes microRNA-mediated silencing", as cited below [1]. The antibody was also validated for Western blot in another article "Reactivity of human AGO2 monoclonal antibody 11A9 with the SWI/SNF complex: A case study for rigorously defining antibody selectivity" [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Sigma
Antibody: AGO2
Catalog number: SAB4200085
Summary: Rat monoclonal IgG2a against a peptide corresponding to amino acids 1-15 of human AGO2. Reacts with rat, human, mouse, bovine, and monkey. Suitable for western blot, immunocytochemistry and immunoprecipitation.
Western blot
Control and AGO2-KO HCT116 cells.
Western blot analysis confirms loss of protein expression in AGO2 HCT116 clonal knockout lines. Courtesy from the Authors [1].
ANKRD52 (A302-372A, Bethyl) is also KO validated in the same Figure. This antibody is a polyclonal antibody, thus the validation data from this article can not be applied to any past or future lots/batches.
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