ADPGK antibody | knockout validation | Abnova H00083440-M01
DOI
//dx.doi.org/10.13070/ko.en.7.2223
Date
2017-04-07

This is a knockout-validated antibody summary, based on the publication "Zinc finger nuclease mediated knockout of ADP-dependent glucokinase in cancer cell lines: effects on cell survival and mitochondrial oxidative metabolism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ADPGK antibody | knockout validation | Abnova H00083440-M01 figure 1
Figure 1. Western blots of HCT116 after transfection with ADPGK ZFNs. GAPDH and actin served as loading controls. From [1].
Antibody information

Mouse monoclonal IgG2a Kappa

Company: Abnova

Antibody: ADPGK

Catalog number: H00083440-M01

Summary: Mouse monoclonal IgG2a Kappa against ADPGK partial recombinant protein (a.a. 425 to 497). Reacts with human, mouse and rat by western blot and ELISA.

Validation Method

Western blot

Sample

Wildtype and ZFN-mediated ADPGK-KO HCT116 and H460 cells. Cell lysates were prepared in RIPA buffer.

Secondary incubation

HRP-coupled anti-mouse antibodies (Santa Cruz Biotechnology, CA).

Detection

Chemiluminescent ECL (SuperSignal West Pico Chemiluminescent Substrate, Pierce, IL) and measured with an LAS-3000 (Fujifilm, Japan).

References
  1. Richter S, Morrison S, Connor T, Su J, Print C, Ronimus R, et al. Zinc finger nuclease mediated knockout of ADP-dependent glucokinase in cancer cell lines: effects on cell survival and mitochondrial oxidative metabolism. PLoS ONE. 2013;8:e65267 pubmed publisher