ADO antibody | knockout validation | Abcam ab134102

This is a knockout-validated antibody summary, based on the publication "Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ADO antibody | knockout validation | Abcam ab134102 figure 1
Figure 1. Endogenous RGS4 and RGS5 proteins in ADO-deficient SH-SY5Y cells (ADO KO); RGS4 and RGS5 are constitutive and insensitive to iron chelators or hypoxia. Please see Courtesy from the Authors [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: ADO

Catalog number: ab134102

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to ADO protein. Reacts with human. Suitable for western blot.

Validation Method

Western blot

Sample

WT and ADO-KO SH-SY5Y cells.

Blocking agent

4% fat free milk in phosphate buffered saline containing 0.1% Tween 20.

Secondary incubation

HRP-conjugated secondary antibodies (DAKO).

Detection

Chemiluminescence substrate (West Dura, 34076, Thermo Fisher Scientific) were used to visualize proteins, using a ChemiDoc XRS+ imaging system (BioRad).

References
  1. Masson N, Keeley T, Giuntoli B, White M, Puerta M, Perata P, et al. Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants. Science. 2019;365:65-69 pubmed publisher