This is a knockout-validated antibody summary, based on the publication "Conserved N-terminal cysteine dioxygenases transduce responses to hypoxia in animals and plants", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG
Company: Abcam
Antibody: ADO
Catalog number: ab134102
Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to ADO protein. Reacts with human. Suitable for western blot.
Western blot
WT and ADO-KO SH-SY5Y cells.
4% fat free milk in phosphate buffered saline containing 0.1% Tween 20.
HRP-conjugated secondary antibodies (DAKO).
Chemiluminescence substrate (West Dura, 34076, Thermo Fisher Scientific) were used to visualize proteins, using a ChemiDoc XRS+ imaging system (BioRad).
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