This is a knockout-validated antibody summary, based on the publication "Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of WT and ADAR1-KO 293T cell clones after mock or IFN-b treatment. Courtesy from the Authors. [1].

Mouse monoclonal IgG1 (lambda light chain)

Company: Santa Cruz

Antibody: ADAR1

Catalog number: sc-271854

Summary: Mouse monoclonal IgG1 (lambda light chain) against amino acids 1051-1226 mapping at the C-terminus of human ADAR1. Recommended for detection of all ADAR1 isoforms of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

WT and ADAR1-KO 293T cells. Cells were directly lysed in NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) with 400mM DTT (Sigma Aldrich).

Blots were developed using Fluorescent (LI-COR, Odyssey CLx) or Chemiluminescent (film) detection.