This is a knockout-validated antibody summary, based on the publication "An acetate switch regulates stress erythropoiesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Abcam
Antibody: ACSS2
Catalog number: ab66038
Summary: Rabbit polyclonal against a synthetic peptide from within residues 650 to the C-terminus of human Acetyl CoA synthetase. Reacts with human and it is predicted to work with mouse and rat. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry (paraffin).
Western blot
Kidney extracts of Acss2 WT or KO mice. Cells were lysed with CytoBuster protein extraction reagent with 1× protease inhibitor cocktail and 1 mM PMSF
1:500 dilution.
Please see Figures 2b, 3e and 3f in the article [1].
Acss2 antibody (Cat. No. 3658, Cell Signaling Technology) is also KO validtaed by IHC.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.