This is a knockout-validated antibody summary, based on two publications "Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis" (western blot knockout validation, see Figure 4f in the article) [1] and "ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition", as cited below [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b (kappa light chain)
Company: Santa Cruz
Antibody: ACSL4
Catalog number: sc-271800
Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 623-675 mapping near the C-terminus of human ACSL4. Recommended for detection of short isoform and long isoform of ACSL4 of mouse, rat, and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including equine.
Western blot
Control and Acsl4−/− mouse embryonic fibroblasts.
1:200 dilution.
Immunoblot analysis of ACSL4 in Acsl4+/+ and Acsl4−/− cells with and without re-expression of ACSL4-Flag. Please see Figure 1d in the article [2].
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