ACSL1 antibody | knockout validation | Cell Signaling 9189

This is a knockout-validated antibody summary, based on the publication "ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: ACSL1

Catalog number: 9189

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ala257 of human ACSL1 protein. Reacts with human, mouse, rat, and monkey. Suitable for by western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and Acsl1−/− mouse embryonic fibroblasts.

Primary incubation

1:1,000 dilution.

Figure

Immunoblot analysis of ACSL1 in WT and Acsl1−/− MEFs cells. Please see Figure S2a in the article [1].

References
  1. Doll S, Proneth B, Tyurina Y, Panzilius E, Kobayashi S, Ingold I, et al. ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition. Nat Chem Biol. 2017;13:91-98 pubmed publisher