This is a knockout-validated antibody summary, based on the publication "Angiotensin-Converting Enzyme 3 (ACE3) Protects Against Pressure Overload-Induced Cardiac Hypertrophy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis for ACE3 in heart tissues of WT and ACE3‐KO mice (n=4 mice in each group). GAPDH is used as loading control. From [1].

Antibody: ACE3

Catalog number: BAM-73-006-EX

Summary: Rabbit polyclonal against ACE3 from mouse origin. Recommended for western blot, immunofluorescence and immunoprecipitation

Company: COSMO BIO

Western blot

Heart tissues of WT and ACE3‐KO mice. Whole‐cell lysates were extracted from ventricles in lysis buffer.

5% fat‐free milk

1:500 dilution, overnight at 4C

peroxidase‐conjugated secondary antibodies for 60 minutes at room temperature

Enhanced chemiluminescence (ECL) detection system (170‐5061, Bio‐Rad), and Bio‐Rad ChemiDoc™ XRS+ (Bio‐Rad)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.