ACAA1 antibody | knockout validation | Cell Signaling 12210

This is a knockout-validated antibody summary, based on the publication "A novel missense variant in ACAA1 contributes to early-onset Alzheimer's disease, impairs lysosomal function, and facilitates amyloid-β pathology and cognitive decline", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

ACAA1 antibody | knockout validation | Cell Signaling 12210 figure 1
Figure 1. Absence of the ACAA1 protein in these ACAA1-KO cells. The β-tubulin was used as the loading control. From [1].
Antibody information

Company: Cell Signaling

Antibody:

Catalog number: 12210

Summary:

Validation Method

Western blot

Sample
Primary incubation

1:1,000 dilution.

Secondary incubation

1:10,000 dilution peroxidase-conjugated anti-mouse antibody (KPL, 474-1806).

References
  1. Luo R, Fan Y, Yang J, Ye M, Zhang D, Guo K, et al. A novel missense variant in ACAA1 contributes to early-onset Alzheimer's disease, impairs lysosomal function, and facilitates amyloid-β pathology and cognitive decline. Signal Transduct Target Ther. 2021;6:325 pubmed publisher