This is a knockout-validated antibody summary, based on the publication "Targeting ABCB1-mediated tumor multidrug resistance by CRISPR/Cas9-based genome editing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: ABCB1

Catalog number: sc-13131

Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 1040-1280 of human Mdr -1 (ABCB1). Recommended for detection of Mdr-1 of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Note: May cross-react with Mdr-3.

Western blot

WT and CRISPR-mediated ABCB1-knockout KBV200 and HCT-8/V cells. Cells were harvested and lysed in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 ng/ml PMSF, 0.03% aprotinin, 1 μM sodium orthovanadate) at 4°C for 30 minutes.

5% BSA.

Horseradish peroxidase-conjugated secondary antibodies.

Chemiluminescent detection reagents and films.

Please see Figure 2a in the article [1].