This is a knockout-validated antibody summary, based on the publication "An ABCA4 loss-of-function mutation causes a canine form of Stargardt disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), and affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. From [1].

Mouse monoclonal IgG1

Company: Novus Biologicals

Antibody: ABCA4

Catalog number: NBP1-30032

Summary: Mouse monoclonal IgG1 against partially purified bovine 220 kDa disc rim protein. Reacts with human, mouse, bovine, canine, feline and Xenopus. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, immunohistochemistry (frozen).

Immunohistochemistry

Control and ABCA4-/- dog retina. Tapetal fundus from the affected male (LAB4), his unaffected heterozygous sibling (LAB6), and an unaffected 10-year-old female Labrador retriever (LAB26) were fixed in 4% PFA in 1x PBS on ice for 15 minutes, washed in 1x PBS for 10 minutes on ice, and cryoprotected in 30% sucrose overnight at 4°C.

1% donkey serum, 0.02% thimerosal, and 0.1% Triton X-100 in 1x PBS) for 30 minutes at room temperature.

1:500 dilution at 4°C overnight.

1:2,000 dilution Alexa 568 secondary antibody (A10037, Invitrogen, Thermo Fisher Scientific) solution for at least 2 hours at room temperature.

Fluorescence images were captured using a Zeiss Axioplan 2 microscope equipped with an AxioCam HRc camera.

The same clone (3F4) is sold as Novus Biologicals NBP1-30032; OriGene TA309076; Santa Cruz Biotechnology sc-65672; LifeSpan Biosciences LS-C150327; Abnova MAB13969; Abcam ab77285; Rockland Immunochemicals 200-301-D05; Invitrogen MA5-33095; MyBioSource MBS500001.