This is a knockout-validated antibody summary, based on the publication "CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunofluorescence of 53BP1 (in red) in control (Cas9 only) and 53BP1-KO (sg53BP1) HeLa cells treated with 1 μM camptothecin for 2 hours. Scale bar, 10 μm. From [1].

Rabbit polyclonal IgG

Company: Novus

Antibody: 53BP1

Catalog number: NB100-304

Summary: Rabbit polyclonal IgG against an epitope mapping between residues 350 and 400 of human 53BP1. Reacts with human, mouse, rat, fish, goat and primate. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (frozen and paraffin), ChIP and flow cytometry.

Immunofluorescence

Control and 53BP1-KO HeLa cells. Cells grown overnight on coverslips were fixed in 4% paraformaldehyde and then permeabilized with 0.5% Triton X-100 plus 300 mM sucrose.

Texas red–conjugated goat anti-rabbit (T-2767, Life Technologies).

Olympus FV1000 confocal microscope.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.