This is a knockout-validated antibody summary, based on the publication "CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of 53BP1 in control (Cas9 only) and 53BP1-KO (sg53BP1) HeLa cells. Actin was used as loading control. The symbol * indicates long exposure. From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: 53BP1

Catalog number: ab21083

Summary: Rabbit polyclonal IgG against a synthetic peptide corresponding to the carboxy-terminus of h53BP1. Reacts with human and mouse. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and in situ hybridization.

Western blot

Control and 53BP1-KO HeLa cells.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.