52 kDa Ro/SSA antibody | knockout validation | Santa Cruz sc-25351

This is a knockout-validated antibody summary, based on the publication "Complement C4 Prevents Viral Infection through Capsid Inactivation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

52 kDa Ro/SSA antibody | knockout validation | Santa Cruz sc-25351 figure 1
Figure 1. Western blot showing TRIM21 KO in 293T and HeLa cells (generated by CRISPR/Cas9). From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: 52 kDa Ro/SSA

Catalog number: sc-25351

Summary: Mouse monoclonal IgG1 κ against amino acids 141-280 of human 52 kDa Ro/SSA (TRIM21). Reacts with human by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

WT and TRIM21 KO 293T and HeLa cells.

Blocking agent

5% milk (Marvel) diluted in PBS+0.01% Tween (PBST).

Primary incubation

0.4μg/mL in 5% milk in PBST.

Secondary incubation

HRP-coupled secondary antibody diluted in 5% milk in PBST.

Detection

ECL Prime Western Blotting Detection Reagent (GE Healthcare).

References
  1. Bottermann M, Foss S, Caddy S, Clift D, van Tienen L, Vaysburd M, et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe. 2019;25:617-629.e7 pubmed publisher