This is a knockout-validated antibody summary, based on the publication "Translational control of nociception via 4E-binding protein 1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of 4E-BP1 in spinal cord from Eif4ebp1-/- and wild-type mice (WT). Beta-actin serves as loading control. From [1].

Antibody: 4E-BP1

Catalog number: 9644

Summary: Rabbit monoclonal raised against a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Detects human, mouse, rat, monkey proteins in western blotting, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry, and flow cytometry.

Company: Cell Signaling Technology

Western blot

Spinal cord of Eif4ebp1-/- and wild-type mice (WT) treated with 4EGI-1 (10 ug, i.t. once a day for 3 days) or vehicle. Ice-cold homogenization buffer containing (in mM): 20 Tris-HCl, pH 7.4; 150 NaCl; 1 EDTA; 1% Triton, 5 NaF; 1.5 Na3VO4, and protease inhibitor cocktail (complete, EDTA-free, Roche Applied Science).

5% dry milk powder in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 hr

1:1000 dilution, overnight

1 h with HRP-conjugated secondary antibody,

Enhanced Chemiluminescence reagent (Perkin Elmer), and exposed to an autoradiography film (Denville Scientific Inc.).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.