This is a knockout-validated antibody summary, based on the publications "Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2", as cited below [1] (see below), "4E-BP1 Is a Tumor Suppressor Protein Reactivated by mTOR Inhibition in Head and Neck Cancer" for immunohistochemistry knockout validation (see [2] figure 6d), and "mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation" for western blot knockout validation (figure 3i) [3], "4E-BP2-dependent translation in parvalbumin neurons controls epileptic seizure threshold" for western blot knockout validation (figure 1h) [4], and "mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation" for western blot knockout validation (figure 3i) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of 4E-BP1 in WT and two independent Eif4ebp1/2 DKO mESCs clones (2 and 5). Courtesy of the Authors.

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: 4E-BP1

Catalog number: 9644

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/ immunocytochemistry and flow cytometry.

Western blot

WT and Eif4ebp1/2 double knockout (DKO) mESCs.