antibody

WNK4 (With no Lysine K, WNK lysine deficient protein kinase 4, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4, Serine/threonine-protein kinase WNK4)

W2999-04

100 ul

polyclonal

domestic rabbit

nonconjugated

WNK4 (With no Lysine K, WNK lysine deficient protein kinase 4, PHA2B, PRKWNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4, Serine/threonine-protein kinase WNK4)

rabbit

Pab

Antibodies

100 ul

IgG

WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been identified in humans (WNK1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is spe-cifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Ca Hu Mo Rt

Synthetic peptide corresponding to residues surrounding Lys59 of human WNK4. Species sequence homology: bovine (100%)

Recongnizes endogenous levels of human total WNK4 protein. Species Crossreactivity: canine, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Lys59 of human WNK4. Species sequence homology: bovine (100%) Specificity: Recongnizes endogenous levels of human total WNK4 protein. Species Crossreactivity: canine, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Veríssimo, F. and Jordan, P. (2001) Oncogene 20, 5562-9, 2. Xu, B. et al. (2000) J Biol Chem 275, 16795-801, 3. Choate, K.A. et al. (2003) Proc Natl Acad Sci U S A 100, 663-8, 4. Wilson, F.H. et al. (2001) Science 293, 1107-12, 5. Zambrowicz, B.P. et al. (2003) Proc Natl Acad Sci U S A 100, 14109-14, 6. Vitari, A.C. et al. (2004) Biochem J 378, 257-68, 7. Xu, B.E. et al. (2002) J Biol Chem 277, 48456-62.

https://usbio-images.r.worldssl.net/prodimages/43/W2999-04_1.jpg