antibody

Shh (Sonic HedgeHog)

S1012-96E

100 ug

monoclonal

rat

nonconjugated

15j188

Shh (Sonic HedgeHog)

rat

Mab

Antibodies

100 ug

15j188

IgG2a

Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for rmShh C-terminal peptide is approximately 1ng/well. Western Blot: 1-2ug/ml with the appropriate secondary reagents to detect mouse Shh C-terminal peptide. The detection limit for recombinant mouse Shh C-terminal peptide is approximately 0.5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mo

Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; Cys199-Ser437)

Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not crossreact with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide.

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Supplied as a liquid in PBS, pH 7.2, 5% trehalose.

~1mg/ml

Product Type: Mab Isotype: IgG2a Clone No: 15j188 Host: rat Source: mouse Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 5% trehalose. Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Immunogen: Purified, E. coli-derived, recombinant mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; Cys199-Ser437) Specificity: Recognizes mouse Shh C-terminal peptide. In direct ELISA, does not crossreact with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide or rmShh N-terminal peptide. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

N WB

-20°C