antibody

MDR2 (MRP2, Multi Drug Resistance Associated Protein, cMOAT, Canalicular Multi Organic Anion Transporter)

M2825-17G

100 ul

polyclonal

domestic rabbit

nonconjugated

MDR2 (MRP2, Multi Drug Resistance Associated Protein, cMOAT, Canalicular Multi Organic Anion Transporter)

rabbit

Pab

Antibodies

100 ul

IgG

Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family (1,2). The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs (2). MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate (3-5). MRP2 is predominately expressed on the apical membranes in the liver (6-9) and kidney proximal tubules (10). It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes (11-13). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence (IF-IC): 1:800 Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to residues surrounding Arg260 of human MDR2 protein.

Recognizes endogenous levels of total human MDR2 at >200kD.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Arg260 of human MDR2 protein. Specificity: Recognizes endogenous levels of total human MDR2 at >200kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF IP WB

-20°C

1. Keppler, D. and Konig, J., FASEB J. 11: 509-516 (1997). 2. Borst, P., et al., J. Natl. Cancer Inst. 92: 1295-1302 (2000). 3. Taniguchi, K., et al., Cancer Res. 56: 4124-4129 (1996). 4. Hooijberg, J.H., et al., Cancer Res. 59: 2532-2535 (1999). 5. Cui, Y., et al., Mol. Pharmacol. 55: 929-937 (1999). 6. Büchler, M., et al., J. Biol. Chem. 271: 15,091-15,098 (1996). 7. Paulusma, C.C., et al., Science 271: 1126-1128 (1996). 8. Mayer, R., et al., J. Cell Biol. 131: 137-150 (1995). 9. Ito, K., et al., J. Biol. Chem. 273: 1684-1688 (1998). 10. Schaub, T.P., et al., J. Am. Soc. Nephrol. 8: 1213-1221 (1997). 11. Dubin, I.N. and Johnson, F.B., Medicine (Baltimore) 33: 155-197 (1954). 12. Kartenbeck, J., et al., Hepatology 23: 1061-1066 (1996). 13. Paulusma, C.C., et al., Hepatology 25: 1539-1542 (1997).

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