DNA-PK Monoclonal Antibody (25-4) | Invitrogen MA5-13244 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DNA-PK Monoclonal Antibody (25-4)

catalog :

MA5-13244

quantity :

500µL

price :

US 349.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

25-4

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, chromatin immunoprecipitation, blocking or activating experiments

citations: 48

Published Application/Species/Dilution	Reference
western blot; human; fig 5	Huguet F, Fernet M, Giocanti N, Favaudon V, Larsen A. Afatinib, an Irreversible EGFR Family Inhibitor, Shows Activity Toward Pancreatic Cancer Cells, Alone and in Combination with Radiotherapy, Independent of KRAS Status. Target Oncol. 2016;11:371-81 pubmed publisher
immunoprecipitation; human; 10 ug	Bombarde O, Boby C, Gomez D, Frit P, Giraud-Panis M, Gilson E, et al. TRF2/RAP1 and DNA-PK mediate a double protection against joining at telomeric ends. EMBO J. 2010;29:1573-84 pubmed publisher
	Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, et al. An in vitro model of murine middle ear epithelium. Dis Model Mech. 2016;9:1405-1417 pubmed
	Sawchuk D, Mansilla-Soto J, Alarcon C, Singha N, Langen H, Bianchi M, et al. Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule. J Biol Chem. 2004;279:29821-31 pubmed
	Johns T, McKay M, Cvrljevic A, Gan H, Taylor C, Xu H, et al. MAb 806 enhances the efficacy of ionizing radiation in glioma xenografts expressing the de2-7 epidermal growth factor receptor. Int J Radiat Oncol Biol Phys. 2010;78:572-8 pubmed publisher
	Papeta N, Zheng Z, Schon E, Brosel S, Altintas M, Nasr S, et al. Prkdc participates in mitochondrial genome maintenance and prevents Adriamycin-induced nephropathy in mice. J Clin Invest. 2010;120:4055-64 pubmed publisher
	Kang K, Zhu C, Wong Y, Gao Q, Ty A, Wong M. Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair. Int J Radiat Oncol Biol Phys. 2012;83:e43-52 pubmed publisher
	Jiang X, Sun Y, Chen S, Roy K, Price B. The FATC domains of PIKK proteins are functionally equivalent and participate in the Tip60-dependent activation of DNA-PKcs and ATM. J Biol Chem. 2006;281:15741-6 pubmed
	Yasaei H, Slijepcevic P. Defective Artemis causes mild telomere dysfunction. Genome Integr. 2010;1:3 pubmed publisher
	Cardinale A, Racaniello M, Saladini S, De Chiara G, Mollinari C, De Stefano M, et al. Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity. J Biol Chem. 2012;287:2618-31 pubmed publisher
	Song S, Lu Y, Choi Y, Han Y, Tang Q, Zhao G, et al. DNA-dependent PK inhibits adeno-associated virus DNA integration. Proc Natl Acad Sci U S A. 2004;101:2112-6 pubmed
	Brown K, Lataxes T, Shangary S, Mannino J, Giardina J, Chen J, et al. Ionizing radiation exposure results in up-regulation of Ku70 via a p53/ataxia-telangiectasia-mutated protein-dependent mechanism. J Biol Chem. 2000;275:6651-6 pubmed
	Ruiz-Herrera A, Smirnova A, Khouriauli L, Nergadze S, Mondello C, Giulotto E. Gene amplification in human cells knocked down for RAD54. Genome Integr. 2011;2:5 pubmed publisher
	Luo M, Rosenke K, Czornak K, Fortunato E. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol. 2007;81:1934-50 pubmed
	Choi Y, Nash K, Byrne B, Muzyczka N, Song S. The effect of DNA-dependent protein kinase on adeno-associated virus replication. PLoS ONE. 2010;5:e15073 pubmed publisher
	Shackelford D. DNA end joining activity is reduced in Alzheimer's disease. Neurobiol Aging. 2006;27:596-605 pubmed
	Mauro M, Rego M, Boisvert R, Esashi F, Cavallo F, Jasin M, et al. p21 promotes error-free replication-coupled DNA double-strand break repair. Nucleic Acids Res. 2012;40:8348-60 pubmed
	Velkova A, Carvalho M, Johnson J, Tavtigian S, Monteiro A. Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair. Cell Cycle. 2010;9:1421-33 pubmed
	Holgersson A, Erdal H, Nilsson A, Lewensohn R, Kanter L. Expression of DNA-PKcs and Ku86, but not Ku70, differs between lymphoid malignancies. Exp Mol Pathol. 2004;77:1-6 pubmed
	Daya S, Cortez N, Berns K. Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway. J Virol. 2009;83:11655-64 pubmed publisher
	Lebrasseur N, Cote G, Miller T, Fielding R, Sawyer D. Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle. Am J Physiol Cell Physiol. 2003;284:C1149-55 pubmed
	Poinsignon C, Moshous D, Callebaut I, de Chasseval R, Villey I, de Villartay J. The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination. J Exp Med. 2004;199:315-21 pubmed
	Wang H, Perrault A, Takeda Y, Qin W, Wang H, Iliakis G. Biochemical evidence for Ku-independent backup pathways of NHEJ. Nucleic Acids Res. 2003;31:5377-88 pubmed
	Calsou P, Delteil C, Frit P, Drouet J, Salles B. Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment. J Mol Biol. 2003;326:93-103 pubmed
	Choul-Li S, Drobecq H, Aumercier M. DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms. Biochem Biophys Res Commun. 2009;390:839-44 pubmed publisher
	Chen L, Trujillo K, Sung P, Tomkinson A. Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase. J Biol Chem. 2000;275:26196-205 pubmed
	Javvadi P, Makino H, Das A, Lin Y, Chen D, Chen B, et al. Threonine 2609 phosphorylation of the DNA-dependent protein kinase is a critical prerequisite for epidermal growth factor receptor-mediated radiation resistance. Mol Cancer Res. 2012;10:1359-68 pubmed publisher
	Yu X, Wang H, Wang P, Chen B, Wang Y. The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival. J Cell Physiol. 2011;226:369-74 pubmed publisher
	Schild-Poulter C, Su A, Shih A, Kelly O, Fritzler M, Goldstein R, et al. Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases. Rheumatology (Oxford). 2008;47:165-71 pubmed publisher
	Zhong X, Safa A. Phosphorylation of RNA helicase A by DNA-dependent protein kinase is indispensable for expression of the MDR1 gene product P-glycoprotein in multidrug-resistant human leukemia cells. Biochemistry. 2007;46:5766-75 pubmed
	Jhappan C, Yusufzai T, Anderson S, Anver M, Merlino G. The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase. Mol Cell Biol. 2000;20:4075-83 pubmed
	Perrault R, Wang H, Wang M, Rosidi B, Iliakis G. Backup pathways of NHEJ are suppressed by DNA-PK. J Cell Biochem. 2004;92:781-94 pubmed
	Mo X, Dynan W. Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites. Mol Cell Biol. 2002;22:8088-99 pubmed
	Ma Y, Pannicke U, Schwarz K, Lieber M. Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination. Cell. 2002;108:781-94 pubmed
	Loong S, Korzh S, Price A. Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis. Oncogene. 2004;23:5562-6 pubmed
	Xiong H, Lee R, Haura E, Edwards J, Dynan W, Li S. Intranuclear delivery of a novel antibody-derived radiosensitizer targeting the DNA-dependent protein kinase catalytic subunit. Int J Radiat Oncol Biol Phys. 2012;83:1023-30 pubmed publisher
	Elliott S, Crawford C, Mulligan E, Summerfield G, Newton P, Wallis J, et al. Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia. Br J Haematol. 2011;152:61-71 pubmed publisher
	Udayakumar D, Bladen C, Hudson F, Dynan W. Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. J Biol Chem. 2003;278:41631-5 pubmed
	Giffin W, Gong W, Schild-Poulter C, Hache R. Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription. Mol Cell Biol. 1999;19:4065-78 pubmed
	Takai H, Xie Y, de Lange T, Pavletich N. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev. 2010;24:2019-30 pubmed publisher
	Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver D, Alt F. A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity. 1998;9:367-76 pubmed
	Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Gueret M. Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing. Nucleic Acids Res. 2003;31:6272-82 pubmed
	Willmore E, Elliott S, Mainou-Fowler T, Summerfield G, Jackson G, O'Neill F, et al. DNA-dependent protein kinase is a therapeutic target and an indicator of poor prognosis in B-cell chronic lymphocytic leukemia. Clin Cancer Res. 2008;14:3984-92 pubmed publisher
	Lau A, Swinbank K, Ahmed P, Taylor D, Jackson S, Smith G, et al. Suppression of HIV-1 infection by a small molecule inhibitor of the ATM kinase. Nat Cell Biol. 2005;7:493-500 pubmed
	Friesland S, Kanter-Lewensohn L, Tell R, Munck-Wikland E, Lewensohn R, Nilsson A. Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy. Head Neck. 2003;25:313-21 pubmed
	Shao R, Cao C, Zhang H, Kohn K, Wold M, Pommier Y. Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes. EMBO J. 1999;18:1397-406 pubmed
	Cho N, Cordon-Cardo C, Li G, Kim S. Allotype imbalance or microsatellite mutation in low-grade soft tissue sarcomas of the extremities in adults. J Pathol. 2002;198:21-9 pubmed
	Vogel R, Seyffert M, Strasser R, de Oliveira A, Dresch C, Glauser D, et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol. 2012;86:143-55 pubmed publisher

product information

Product Type :

Antibody

Product Name :

DNA-PK Monoclonal Antibody (25-4)

Catalog # :

MA5-13244

Quantity :

500µL

Price :

US 349.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Human

Applications :

Gel Shift: Assay Dependent, Western Blot: 1:1000

Species :

Human

Clone :

25-4

Isotype :

IgG2a

Storage :

4° C

Description :

DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks

Immunogen :

Human DNA-dependent Protein Kinase purified from HeLa cells

Format :

Liquid

Applications w/Dilutions :

Gel Shift: Assay Dependent, Western Blot: 1:1000

Aliases :

DNA-PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, hyper-radiosensitivity of murine scid mutation, complementing 1, p460

PubMed References :

10064605, 10330147, 10692474, 10805749, 10854421, 11955432, 12210059, 12391174, 12519750, 12547193, 12658736, 12917393, 12954774, 14576316, 14744996, 14766968, 15123719, 15184871, 15211575, 15215044, 15834407, 15908050, 16603769, 17151099, 17441731, 18208821, 18559621, 19759155, 19836356, 20305393, 20407424, 20638193, 20665702, 20678254, 20801936, 20978358, 21083655, 21188139, 21418575, 22013059, 22138455, 22139836, 22516386, 22735704, 9768756