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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
6x-His Tag Monoclonal Antibody (HIS.H8), HRP
catalog :
MA1-21315-HRP
quantity :
50 µL
price :
US 225.00
clonality :
monoclonal
host :
mouse
conjugate :
HRP
clone name :
HIS.H8
application :
western blot
more info or order :
Invitrogen product webpage
citations: 17
Reference
Chi X, Liu X, Wang C, Zhang X, Li X, Hou J, et al. Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain. Nat Commun. 2020;11:4528 pubmed publisher
Cao Y, Su B, Guo X, Sun W, Deng Y, Bao L, et al. Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells. Cell. 2020;182:73-84.e16 pubmed publisher
Sumrall E, Shen Y, Keller A, Rismondo J, Pavlou M, Eugster M, et al. Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion. PLoS Pathog. 2019;15:e1008032 pubmed publisher
Szwedziak P, Pilhofer M. Bidirectional contraction of a type six secretion system. Nat Commun. 2019;10:1565 pubmed publisher
Pollak A, Liu C, Gudlur A, Mayfield J, Dalton N, Gu Y, et al. A secretory pathway kinase regulates sarcoplasmic reticulum Ca2+ homeostasis and protects against heart failure. elife. 2018;7: pubmed publisher
Hrit J, Goodrich L, Li C, Wang B, Nie J, Cui X, et al. OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development. elife. 2018;7: pubmed publisher
Abouelkhair M, Bemis D, Giannone R, Frank L, Kania S. Characterization of a leukocidin identified in Staphylococcus pseudintermedius. PLoS ONE. 2018;13:e0204450 pubmed publisher
Paul S, Dansithong W, Figueroa K, Scoles D, Pulst S. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun. 2018;9:3648 pubmed publisher
McAteer S, Sy B, Wong J, Tollervey D, Gally D, Tree J. Ribosome maturation by the endoribonuclease YbeY stabilizes a type 3 secretion system transcript required for virulence of enterohemorrhagic Escherichia coli. J Biol Chem. 2018;293:9006-9016 pubmed publisher
Yang B, Tang S, Ma C, Li S, Shao G, Dang B, et al. Spontaneous and specific chemical cross-linking in live cells to capture and identify protein interactions. Nat Commun. 2017;8:2240 pubmed publisher
He K, Marsland Iii R, Upadhyayula S, Song E, Dang S, Capraro B, et al. Dynamics of phosphoinositide conversion in clathrin-mediated endocytic traffic. Nature. 2017;: pubmed publisher
Lee M, Shei W, Chan A, Chua B, Goh S, Chong Y, et al. Primary angle closure glaucoma (PACG) susceptibility gene PLEKHA7 encodes a novel Rac1/Cdc42 GAP that modulates cell migration and blood-aqueous barrier function. Hum Mol Genet. 2017;26:4011-4027 pubmed publisher
Gontarek B, Neelakandan A, Wu H, Becraft P. NKD Transcription Factors Are Central Regulators of Maize Endosperm Development. Plant Cell. 2016;28:2916-2936 pubmed publisher
Lei S, Ramesh A, Twitchell E, Wen K, Bui T, Weiss M, et al. High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs. Front Microbiol. 2016;7:1699 pubmed
Dormeshkin D, Gilep A, Sergeev G, Usanov S. Development of CYB5-fusion monitoring system for efficient periplasmic expression of multimeric proteins in Escherichia coli. Protein Expr Purif. 2016;128:60-6 pubmed publisher
Sokolowski M, DeFreece C, Servant G, Kines K, deHaro D, Belancio V. Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein. Mob DNA. 2014;5:29 pubmed publisher
LEE SOETY J, Jones J, Macgibeny M, Remaly E, Daniels L, Ito A, et al. Yeast hnRNP-related proteins contribute to the maintenance of telomeres. Biochem Biophys Res Commun. 2012;426:12-7 pubmed publisher
image
image 1 :
Invitrogen MA1-21315-HRP image 1
Western blot analysis of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-HRP) at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
product information
Product Type :
Antibody
Product Name :
6x-His Tag Monoclonal Antibody (HIS.H8), HRP
Catalog # :
MA1-21315-HRP
Quantity :
50 µL
Price :
US 225.00
Clonality :
Monoclonal
Purity :
protein A
Host :
Mouse
Reactivity :
Tag
Applications :
Western Blot: 1:500
Species :
Tag
Clone :
HIS.H8
Isotype :
IgG2b
Storage :
4° C
Description :
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
Immunogen :
6x His synthetic peptide.
Format :
Liquid
Applications w/Dilutions :
Western Blot: 1:500
Aliases :
6His tag; 6X His; His Tag; Histidine Tag
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA