product summary
request information :
company name :
Thermo Scientific Pierce Products
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, Arcturus, Perseptive Biosystems
product type :
antibody
product name :
Estrogen Receptor Monoclonal Antibody (TE111.5D11)
catalog :
MA1-12692
quantity :
100 µg
price :
US 455
clonality :
monoclonal
host :
mouse
reactivity :
mouse, pig, Chinese hamster, sheep, rabbit, cow, dog, rat, human, chicken
publications citing this reagent :
total: 43
  1. IHC(4 ug/ml)
    González-Morán M. Changes in the cellular localization of estrogen receptor alpha in the growing and regressing ovaries of Gallus domesticus during development. Biochem Biophys Res Commun. 2014;447:197-204 pubmed publisher
  2. ChIP
    Gagnidze K, Weil Z, Faustino L, Schaafsma S, Pfaff D. Early histone modifications in the ventromedial hypothalamus and preoptic area following oestradiol administration. J Neuroendocrinol. 2013;25:939-55 pubmed publisher
  3. EMSA
    Kruer T, Cummins T, Powell D, Wittliff J. Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior. Clin Biochem. 2013;46:1739-46 pubmed publisher
  4. ChIP
    Miranda T, Voss T, Sung M, Baek S, John S, HAWKINS M, et al. Reprogramming the chromatin landscape: interplay of the estrogen and glucocorticoid receptors at the genomic level. Cancer Res. 2013;73:5130-9 pubmed publisher
  5. ChIP
    Bhat-Nakshatri P, Song E, Collins N, Uversky V, Dunker A, O'MALLEY B, et al. Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing. BMC Med Genomics. 2013;6:21 pubmed publisher
  6. WB(1:200)
    Arao Y, Hamilton K, Coons L, Korach K. Estrogen receptor α L543A,L544A mutation changes antagonists to agonists, correlating with the ligand binding domain dimerization associated with DNA binding activity. J Biol Chem. 2013;288:21105-16 pubmed publisher
  7. ChIP
    Maethner E, García-Cuéllar M, Breitinger C, Takacova S, Divoky V, Hess J, et al. MLL-ENL inhibits polycomb repressive complex 1 to achieve efficient transformation of hematopoietic cells. Cell Rep. 2013;3:1553-66 pubmed publisher
  8. ChIP
    Kharbanda A, Rajabi H, Jin C, Raina D, Kufe D. Oncogenic MUC1-C promotes tamoxifen resistance in human breast cancer. Mol Cancer Res. 2013;11:714-23 pubmed publisher
  9. IHC
    Katayama M, Fukuda T, Narabara K, Abe A, Kondo Y. Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis. Biosci Biotechnol Biochem. 2012;76:2003-7 pubmed
  10. ChIP(1:35)
    Privette Vinnedge L, Ho S, Wikenheiser-Brokamp K, Wells S. The DEK oncogene is a target of steroid hormone receptor signaling in breast cancer. PLoS ONE. 2012;7:e46985 pubmed publisher
image
image 1 :
Thermo Scientific Pierce Products MA1-12692 image 1
product information
Product Type :
MA1-12692
Product Name :
Estrogen Receptor Monoclonal Antibody (TE111.5D11)
Catalog # :
MA1-12692
Quantity :
100 µg
Price :
US 455
Clonality :
Monoclonal
Purity :
Protein A/G
Host :
Mouse
Reactivity :
Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat
Applications :
Blocking Assay, ChIP assay, Gel Shift, Immunoprecipitation and Western Blot
Species :
Bv, Ca, Ck, Hm, Hu, Ms, Ov, Po, Rb, Rt
Clone :
TE111.5D11
Gene Identification :
Entrez: 407238, 403640, 396099, 100768474, 2099, 13982, 443228, 397435, 100351261, 24890; Uniprot: P49884, P06212, P03372, P19785, Q29040, P06211
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
The estrogen receptor (ER) gene consists of more than 140 kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Immunogen :
C-terminal fragment (aa 302-595) of human ER expressed in E coli.
Format :
Liquid; PBS with 0.08 % sodium azide
Applications w/Dilutions :
BLOCK Assay Dependent, GS Assay Dependent, ChIP Assay Dependent, WB 1-2 µg/ml, IP 2 µg/ml protein lysate
Aliases :
ER
PubMed References :
17671196, 18348185, 18219273, 9832502, 11280746, 11563984, 12915759, 14695898, 15205327, 15808510, 16203130, 16971528, 17157980, 19541800, 19809499, 20047966, 20228224, 9271411, 15368364, 16357144, 11476946
publications citing this reagent :
  1. WB(1:100) : IHC(1:100)
    Kam M, Lee K, Tam P, Lui V. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. PLoS ONE. 2012;7:e35799 pubmed publisher
  2. ChIP
    He H, Meyer C, Chen M, Jordan V, Brown M, Liu X. Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics. Genome Res. 2012;22:1015-25 pubmed publisher
  3. WB
    Tuttle R, Miller K, Maiorano J, Termuhlen P, Gao Y, Berberich S. Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence. Int J Cancer. 2012;130:2291-9 pubmed publisher
  4. IP
    Miranda-Carboni G, Guemes M, Bailey S, Anaya E, Corselli M, Peault B, et al. GATA4 regulates estrogen receptor-alpha-mediated osteoblast transcription. Mol Endocrinol. 2011;25:1126-36 pubmed publisher
  5. ChIP
    Zhao L, Glazov E, Pattabiraman D, Al-Owaidi F, Zhang P, Brown M, et al. Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res. 2011;39:4664-79 pubmed publisher
  6. EMSA
    Shu F, Sidell N, Yang D, Kallen C. The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses. J Steroid Biochem Mol Biol. 2010;120:172-9 pubmed publisher
  7. : human (ChIP)
    Badve S, Collins N, Bhat-Nakshatri P, Turbin D, Leung S, Thorat M, et al. Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications. Am J Pathol. 2010;176:2139-49 pubmed publisher
  8. : human (EMSA : ChIP)
    Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed publisher
  9. : human (IC(1:100))
    Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 pubmed publisher
  10. WB
    Flamini M, Sanchez A, Goglia L, Tosi V, Genazzani A, Simoncini T. Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells. Mol Hum Reprod. 2009;15:675-85 pubmed publisher
  11. ChIP
    Tallack M, Keys J, Humbert P, Perkins A. EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2. J Biol Chem. 2009;284:20966-74 pubmed publisher
  12. WB : IC
    Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed publisher
  13. ChIP
    Lupien M, Eeckhoute J, Meyer C, Krum S, Rhodes D, Liu X, et al. Coactivator function defines the active estrogen receptor alpha cistrome. Mol Cell Biol. 2009;29:3413-23 pubmed publisher
  14. EMSA : ChIP
    Wang C, Yu J, Kallen C. Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells. PLoS ONE. 2008;3:e3523 pubmed publisher
  15. WB : IC : IP
    Giretti M, Fu X, De Rosa G, Sarotto I, Baldacci C, Garibaldi S, et al. Extra-nuclear signalling of estrogen receptor to breast cancer cytoskeletal remodelling, migration and invasion. PLoS ONE. 2008;3:e2238 pubmed publisher
  16. WB(1:400) : IC(1:50) : Neut
    Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed publisher
  17. IC
    Krum S, Miranda-Carboni G, Hauschka P, Carroll J, Lane T, Freedman L, et al. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival. EMBO J. 2008;27:535-45 pubmed publisher
  18. ChIP
    Caslini C, Yang Z, El-Osta M, Milne T, Slany R, Hess J. Interaction of MLL amino terminal sequences with menin is required for transformation. Cancer Res. 2007;67:7275-83 pubmed
  19. ChIP
    Tallack M, Keys J, Perkins A. Erythroid Kruppel-like factor regulates the G1 cyclin dependent kinase inhibitor p18INK4c. J Mol Biol. 2007;369:313-21 pubmed
  20. WB(1:300) : ChIP(2 ug)
    Kazi A, Koos R. Estrogen-induced activation of hypoxia-inducible factor-1alpha, vascular endothelial growth factor expression, and edema in the uterus are mediated by the phosphatidylinositol 3-kinase/Akt pathway. Endocrinology. 2007;148:2363-74 pubmed
  21. WB : IP : ChIP
    Hirano S, Furutama D, Hanafusa T. Physiologically high concentrations of 17beta-estradiol enhance NF-kappaB activity in human T cells. Am J Physiol Regul Integr Comp Physiol. 2007;292:R1465-71 pubmed
  22. WB(1:2000) : ChIP(5-10 ug)
    Zhang Z, Teng C. Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression. Mol Cell Endocrinol. 2007;264:128-41 pubmed
  23. ChIP
    Carroll J, Meyer C, Song J, Li W, Geistlinger T, Eeckhoute J, et al. Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006;38:1289-97 pubmed
  24. IP(1:250)
    Mannella P, Brinton R. Estrogen receptor protein interaction with phosphatidylinositol 3-kinase leads to activation of phosphorylated Akt and extracellular signal-regulated kinase 1/2 in the same population of cortical neurons: a unified mechanism of estrogen action. J Neurosci. 2006;26:9439-47 pubmed
  25. WB : IC
    Simoncini T, Scorticati C, Mannella P, Fadiel A, Giretti M, Fu X, et al. Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway. Mol Endocrinol. 2006;20:1756-71 pubmed
  26. WB : IP(1:30)
    Cheng A, Jin V, Fan M, Smith L, Liyanarachchi S, Yan P, et al. Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to estrogen receptor-alpha responsive promoters. Mol Cell. 2006;21:393-404 pubmed
  27. IHC(1:150)
    Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, et al. Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat. Biochem Biophys Res Commun. 2006;341:755-62 pubmed
  28. ChIP
    Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
  29. ChIP
    Jeffy B, Hockings J, Kemp M, Morgan S, Hager J, Beliakoff J, et al. An estrogen receptor-alpha/p300 complex activates the BRCA-1 promoter at an AP-1 site that binds Jun/Fos transcription factors: repressive effects of p53 on BRCA-1 transcription. Neoplasia. 2005;7:873-82 pubmed
  30. IP
    Wessler S, Otto C, Wilck N, Stangl V, Fritzemeier K. Identification of estrogen receptor ligands leading to activation of non-genomic signaling pathways while exhibiting only weak transcriptional activity. J Steroid Biochem Mol Biol. 2006;98:25-35 pubmed
  31. ChIP
    Carroll J, Liu X, Brodsky A, Li W, Meyer C, Szary A, et al. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005;122:33-43 pubmed
  32. IP
    Sentis S, Le Romancer M, Bianchin C, Rostan M, Corbo L. Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity. Mol Endocrinol. 2005;19:2671-84 pubmed
  33. WB : IP : ChIP
    Park K, Krishnan V, O'MALLEY B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
company information
Thermo Scientific Pierce Products
PO Box 117
Rockford, IL 61105
www.pierce-antibodies.com
8008743723
headquarters: USA