product summary
request information :
company name :
Thermo Scientific Pierce Products
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, ABgene, Affinity BioReagents, Nunc
product type :
antibody
product name :
Estrogen Receptor Monoclonal Antibody (TE111.5D11)
catalog :
MA1-12692
quantity :
100 µg
price :
US 455
clonality :
monoclonal
host :
mouse
reactivity :
chicken, human, cow, dog, rat, mouse
application :
western blot, immunoprecipitation, neutralization, chromatin immunoprecipitation
publications citing this reagent :
total: 50
  1. WB(1:100) : IHC(1:100)
    Kam M, Lee K, Tam P, Lui V. Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons. PLoS ONE. 2012;7:e35799 pubmed publisher
  2. IP
    Miranda-Carboni G, Guemes M, Bailey S, Anaya E, Corselli M, Peault B, et al. GATA4 regulates estrogen receptor-alpha-mediated osteoblast transcription. Mol Endocrinol. 2011;25:1126-36 pubmed publisher
  3. ChIP
    Zhao L, Glazov E, Pattabiraman D, Al-Owaidi F, Zhang P, Brown M, et al. Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb. Nucleic Acids Res. 2011;39:4664-79 pubmed publisher
  4. EMSA
    Shu F, Sidell N, Yang D, Kallen C. The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses. J Steroid Biochem Mol Biol. 2010;120:172-9 pubmed publisher
  5. ChIP : human (ChIP)
    Badve S, Collins N, Bhat-Nakshatri P, Turbin D, Leung S, Thorat M, et al. Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications. Am J Pathol. 2010;176:2139-49 pubmed publisher
  6. EMSA : ChIP : human (EMSA : ChIP)
    Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 pubmed publisher
  7. IC(1:100) : human (IC(1:100))
    Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 pubmed
  8. WB
    Flamini M, Sanchez A, Goglia L, Tosi V, Genazzani A, Simoncini T. Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells. Mol Hum Reprod. 2009;15:675-85 pubmed publisher
  9. ChIP
    Tallack M, Keys J, Humbert P, Perkins A. EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2. J Biol Chem. 2009;284:20966-74 pubmed
  10. WB : IC
    Milanesi L, Vasconsuelo A, de Boland A, Boland R. Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue. Steroids. 2009;74:489-97 pubmed publisher
image
image 1 :
Thermo Scientific Pierce Products MA1-12692 image 1
product information
Product Type :
MA1-12692
Product Name :
Estrogen Receptor Monoclonal Antibody (TE111.5D11)
Catalog # :
MA1-12692
Quantity :
100 µg
Price :
US 455
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat
Applications :
Blocking Assay, ChIP assay, Gel Shift, Immunoprecipitation and Western Blot
Species :
Bv, Ca, Ck, Hm, Hu, Ms, Ov, Po, Rb, Rt
Clone :
TE111.5D11
Gene Identification :
Entrez: 24890, 396099, 100351261, 2099, 407238, 13982, 100768474, 443228, 397435, 403640; Uniprot: P06211, P06212, P03372, P49884, P19785, Q29040
Isotype :
IgG1
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
The estrogen receptor (ER) gene consists of more than 140 kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
Immunogen :
C-terminal fragment (aa 302-595) of human ER expressed in E coli.
Format :
Liquid; PBS with 0.08 % sodium azide
Applications w/Dilutions :
WB 1-2 µg/ml, IP 2 µg/ml protein lysate, BLOCK Assay Dependent, GS Assay Dependent, ChIP Assay Dependent
Aliases :
ER
PubMed References :
9832502, 11280746, 11563984, 12915759, 12915759, 14695898, 15205327, 15808510, 15808510, 15808510, 16203130, 16971528, 17157980, 17157980, 19541800, 19809499, 20047966, 20047966, 20228224
publications citing this reagent :
  1. ChIP
    Lupien M, Eeckhoute J, Meyer C, Krum S, Rhodes D, Liu X, et al. Coactivator function defines the active estrogen receptor alpha cistrome. Mol Cell Biol. 2009;29:3413-23 pubmed publisher
  2. EMSA : ChIP
    Wang C, Yu J, Kallen C. Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells. PLoS ONE. 2008;3:e3523 pubmed
  3. WB : IC : IP
    Giretti M, Fu X, De Rosa G, Sarotto I, Baldacci C, Garibaldi S, et al. Extra-nuclear signalling of estrogen receptor to breast cancer cytoskeletal remodelling, migration and invasion. PLoS ONE. 2008;3:e2238 pubmed publisher
  4. WB(1:400) : IC(1:50) : Neut
    Milanesi L, Russo de Boland A, Boland R. Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line. J Cell Biochem. 2008;104:1254-73 pubmed
  5. IC
    Krum S, Miranda-Carboni G, Hauschka P, Carroll J, Lane T, Freedman L, et al. Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival. EMBO J. 2008;27:535-45 pubmed publisher
  6. ChIP
    Caslini C, Yang Z, El-Osta M, Milne T, Slany R, Hess J. Interaction of MLL amino terminal sequences with menin is required for transformation. Cancer Res. 2007;67:7275-83 pubmed
  7. ChIP
    Tallack M, Keys J, Perkins A. Erythroid Kruppel-like factor regulates the G1 cyclin dependent kinase inhibitor p18INK4c. J Mol Biol. 2007;369:313-21 pubmed
  8. WB(1:300) : ChIP(2 ug)
    Kazi A, Koos R. Estrogen-induced activation of hypoxia-inducible factor-1alpha, vascular endothelial growth factor expression, and edema in the uterus are mediated by the phosphatidylinositol 3-kinase/Akt pathway. Endocrinology. 2007;148:2363-74 pubmed
  9. WB : IP : ChIP
    Hirano S, Furutama D, Hanafusa T. Physiologically high concentrations of 17beta-estradiol enhance NF-kappaB activity in human T cells. Am J Physiol Regul Integr Comp Physiol. 2007;292:R1465-71 pubmed
  10. WB(1:2000) : ChIP(5-10 ug)
    Zhang Z, Teng C. Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression. Mol Cell Endocrinol. 2007;264:128-41 pubmed
  11. ChIP
    Carroll J, Meyer C, Song J, Li W, Geistlinger T, Eeckhoute J, et al. Genome-wide analysis of estrogen receptor binding sites. Nat Genet. 2006;38:1289-97 pubmed
  12. IP(1:250)
    Mannella P, Brinton R. Estrogen receptor protein interaction with phosphatidylinositol 3-kinase leads to activation of phosphorylated Akt and extracellular signal-regulated kinase 1/2 in the same population of cortical neurons: a unified mechanism of estrogen action. J Neurosci. 2006;26:9439-47 pubmed
  13. WB : IC
    Simoncini T, Scorticati C, Mannella P, Fadiel A, Giretti M, Fu X, et al. Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway. Mol Endocrinol. 2006;20:1756-71 pubmed
  14. WB : IP(1:30)
    Cheng A, Jin V, Fan M, Smith L, Liyanarachchi S, Yan P, et al. Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to estrogen receptor-alpha responsive promoters. Mol Cell. 2006;21:393-404 pubmed
  15. IHC(1:150)
    Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, et al. Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat. Biochem Biophys Res Commun. 2006;341:755-62 pubmed
  16. ChIP
    Milne T, Martin M, Brock H, Slany R, Hess J. Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications. Cancer Res. 2005;65:11367-74 pubmed
  17. ChIP
    Jeffy B, Hockings J, Kemp M, Morgan S, Hager J, Beliakoff J, et al. An estrogen receptor-alpha/p300 complex activates the BRCA-1 promoter at an AP-1 site that binds Jun/Fos transcription factors: repressive effects of p53 on BRCA-1 transcription. Neoplasia. 2005;7:873-82 pubmed
  18. IP
    Wessler S, Otto C, Wilck N, Stangl V, Fritzemeier K. Identification of estrogen receptor ligands leading to activation of non-genomic signaling pathways while exhibiting only weak transcriptional activity. J Steroid Biochem Mol Biol. 2006;98:25-35 pubmed
  19. ChIP
    Carroll J, Liu X, Brodsky A, Li W, Meyer C, Szary A, et al. Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1. Cell. 2005;122:33-43 pubmed
  20. IP
    Sentis S, Le Romancer M, Bianchin C, Rostan M, Corbo L. Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity. Mol Endocrinol. 2005;19:2671-84 pubmed
  21. WB : IP : ChIP
    Park K, Krishnan V, O'MALLEY B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 pubmed
  22. EMSA
    Stokes K, Alston-Mills B, Teng C. Estrogen response element and the promoter context of the human and mouse lactoferrin genes influence estrogen receptor alpha-mediated transactivation activity in mammary gland cells. J Mol Endocrinol. 2004;33:315-34 pubmed
  23. WB
    Capiati D, Rossi A, Picotto G, Benassati S, Boland R. Inhibition of serum-stimulated mitogen activated protein kinase by 1alpha,25(OH)2-vitamin D3 in MCF-7 breast cancer cells. J Cell Biochem. 2004;93:384-97 pubmed
  24. WB
    Kirma N, Luthra R, Jones J, Liu Y, Nair H, Mandava U, et al. Overexpression of the colony-stimulating factor (CSF-1) and/or its receptor c-fms in mammary glands of transgenic mice results in hyperplasia and tumor formation. Cancer Res. 2004;64:4162-70 pubmed
  25. WB
    Flötotto T, Niederacher D, Hohmann D, Heimerzheim T, Dall P, Djahansouzi S, et al. Molecular mechanism of estrogen receptor (ER)alpha-specific, estradiol-dependent expression of the progesterone receptor (PR) B-isoform. J Steroid Biochem Mol Biol. 2004;88:131-42 pubmed
  26. ChIP : mouse (ChIP)
    Letting D, Chen Y, Rakowski C, Reedy S, Blobel G. Context-dependent regulation of GATA-1 by friend of GATA-1. Proc Natl Acad Sci U S A. 2004;101:476-81 pubmed
  27. WB : IC : common platanna (WB : IC)
    Wu K, Tobias M, Kelley D. Estrogen receptor expression in laryngeal muscle in relation to estrogen-dependent increases in synapse strength. Neuroendocrinology. 2003;78:72-80 pubmed
  28. WB : IC(1:50) : IP
    Simoncini T, Rabkin E, Liao J. Molecular basis of cell membrane estrogen receptor interaction with phosphatidylinositol 3-kinase in endothelial cells. Arterioscler Thromb Vasc Biol. 2003;23:198-203 pubmed
  29. IHC
    DiRenzo J, Signoretti S, Nakamura N, Rivera-Gonzalez R, Sellers W, Loda M, et al. Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line. Cancer Res. 2002;62:89-98 pubmed
  30. IHC(4 ug/ml)
    González-Agüero G, Ondarza R, Gamboa-Dominguez A, Cerbon M, Camacho-Arroyo I. Progesterone receptor isoforms expression pattern in human astrocytomas. Brain Res Bull. 2001;56:43-8 pubmed
  31. IP : human (IP)
    Schlegel A, Wang C, Pestell R, Lisanti M. Ligand-independent activation of oestrogen receptor alpha by caveolin-1. Biochem J. 2001;359:203-10 pubmed
  32. WB : IC(1:50)
    Monje P, Zanello S, Holick M, Boland R. Differential cellular localization of estrogen receptor alpha in uterine and mammary cells. Mol Cell Endocrinol. 2001;181:117-29 pubmed
  33. WB
    Biswas D, Cruz A, Pettit N, Mutter G, Pardee A. A therapeutic target for hormone-independent estrogen receptor-positive breast cancers. Mol Med. 2001;7:59-67 pubmed
  34. WB
    Kirma N, Gill K, Mandava U, Tekmal R. Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice. Cancer Res. 2001;61:1910-8 pubmed
  35. EMSA
    Tyulmenkov V, Klinge C. Selectivity of antibodies to estrogen receptors alpha and beta (ERalpha and ERbeta) for detecting DNA-bound ERalpha and ERbeta in vitro. Steroids. 2000;65:505-12 pubmed
  36. WB
    Klinge C. Role of estrogen receptor ligand and estrogen response element sequence on interaction with chicken ovalbumin upstream promoter transcription factor (COUP-TF). J Steroid Biochem Mol Biol. 1999;71:1-19 pubmed
  37. WB : Neut(0.5 ug) : IP
    Biswas D, Reddy P, Pickard M, Makkad B, Pettit N, Pardee A. Calmodulin is essential for estrogen receptor interaction with its motif and activation of responsive promoter. J Biol Chem. 1998;273:33817-24 pubmed
  38. IP
    Zwijsen R, Buckle R, Hijmans E, Loomans C, Bernards R. Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998;12:3488-98 pubmed
  39. IC : EMSA
    Biswas D, Averboukh L, Sheng S, Martin K, Ewaniuk D, Jawde T, et al. Classification of breast cancer cells on the basis of a functional assay for estrogen receptor. Mol Med. 1998;4:454-67 pubmed
  40. WB : IP
    Neuman E, Ladha M, Lin N, Upton T, Miller S, DiRenzo J, et al. Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4. Mol Cell Biol. 1997;17:5338-47 pubmed
company information
Thermo Scientific Pierce Products
PO Box 117
Rockford, IL 61105
www.pierce-antibodies.com
8008743723
headquarters: USA