product summary
request information :
company name :
Thermo Scientific Pierce Products
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, HyClone, ABgene, Open Biosystems, Dharmacon Research, Affinity BioReagents, Nunc
product type :
antibody
product name :
Estrogen Receptor
catalog :
MA1-12692
quantity :
100 µg
price :
449.00 USD
clonality :
monoclonal
host :
mouse
reactivity :
human, cow, dog, rat, mouse
application :
western blot, immunoprecipitation, neutralization, chromatin immunoprecipitation
publications citing this reagent :
  1. ChIP : human (ChIP)
    Badve S, Collins N, Bhat-Nakshatri P, Turbin D, Leung S, Thorat M, et al. Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications. Am J Pathol. 2010;176:2139-49 PMID 20228224
  2. EMSA : ChIP : human (EMSA : ChIP)
    Mason C, Shu F, Wang C, Session R, Kallen R, Sidell N, et al. Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements. Nucleic Acids Res. 2010;38:2355-68 PMID 20047966
  3. IC(1:100) : human (IC(1:100))
    Salama S, Kamel M, Botting S, Salih S, Borahay M, Hamed A, et al. Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells. PLoS ONE. 2009;4:e7356 PMID 19809499
  4. WB : IP : ChIP
    Park K, Park K, Krishnan V, O'MALLEY B, Yamamoto Y, Gaynor R. Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation. Mol Cell. 2005;18:71-82 PMID 15808510
  5. ChIP : mouse (ChIP)
    Blobel G, Rakowski C, Chen Y, Letting D, Reedy S. Context-dependent regulation of GATA-1 by friend of GATA-1. Proc Natl Acad Sci U S A. 2004;101:476-81 PMID 14695898
  6. WB : IC : common platanna (WB : IC)
    Wu K, Tobias M, Kelley D. Estrogen receptor expression in laryngeal muscle in relation to estrogen-dependent increases in synapse strength. Neuroendocrinology. 2003;78:72-80 PMID 12915759
  7. IP : human (IP)
    Schlegel A, Wang C, Pestell R, Lisanti M. Ligand-independent activation of oestrogen receptor alpha by caveolin-1. Biochem J. 2001;359:203-10 PMID 11563984
  8. EMSA
    Tyulmenkov V, Klinge C. Selectivity of antibodies to estrogen receptors alpha and beta (ERalpha and ERbeta) for detecting DNA-bound ERalpha and ERbeta in vitro. Steroids. 2000;65:505-12 PMID 10978729
more info or order :
Thermo Scientific Pierce Products product webpage
image
image 1 :
Thermo Scientific Pierce Products MA1-12692 image 1
product information
Catalog Number :
MA1-12692
product name :
Estrogen Receptor
Long Product Name :
Estrogen Receptor Antibody (TE111.5D11)
product type :
antibody
List Price :
449.00 USD
clonality :
monoclonal
Host Name :
mouse
Clone :
TE111.5D11
Isotype :
IgG1
Product Description :
MA1-12692 detects Estrogen Receptor in Human, Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Pig, Dog and Chicken samples. MA1-12692 has been successfully used in western blot and immunoprecipitation. The MA1-12692 immunogen is C-terminal fragment (aa 302-595) of human ER expressed in E. coli. Positive Control: MCF-7 cells.
CrossReactivity :
Bv, Ca, Ck, Hm, Hu, Ms, Ov, Po, Rb, Rt
SpeciesSummary :
Bovine, Canine, Chicken, Hamster, Human, Mouse, Ovine, Porcine, Rabbit, Rat
Background :
The estrogen receptor (ER) gene consists of more than 140 kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
ALTnames :
ER
Immunogen :
C-terminal fragment (aa 302-595) of human ER expressed in E coli.
buffer :
100 µg purified antibody (1 mg/ml) in PBS with 0.08% sodium azide.
Preservative :
0.08% Sodium azide
Packaging :
100 µg
PackageSize :
100
Concentration :
1 mg/ml
Uses :
BLOCK (Assay Dependent), GS (Assay Dependent), ChIP (Assay Dependent), WB (1-2 µg/ml), IP (2 µg/ml protein lysate)
Application Summary :
BLOCK, ChIP, GS, IP, WB
Storage :
-20° C, Avoid Freeze/Thaw Cycles
more info or order :
Thermo Scientific Pierce Products product webpage
company information
Thermo Scientific Pierce Products
PO Box 117
Rockford, IL 61105
www.pierce-antibodies.com
8008743723
Headquarters: USA