GSK-3beta Antibody | StressMarq Biosciences SPC-169D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

GSK-3beta Antibody

catalog :

SPC-169D

quantity :

100 µg

price :

252.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GSK3beta Polyclonal Antibody (SPC-169) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GSK3beta Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

product information

Catalog No :

SPC-169D

Product Name :

GSK-3beta Antibody

Description :

Rabbit Anti-Human GSK-3beta Polyclonal

Target :

GSK3 Beta

Conjugate :

Unconjugated

2021 List Price :

252.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

Glycogen Synthase kinase 3 beta Antibody, GSK 3 beta Antibody, GSK 3B Antibody, GSK-3 beta Antibody, GSK3B Antibody, GSK3B_Human Antibody, GSK3beta isoform Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta

Immunogen Species :

Human

Accession Number :

NP_001139628.1

Swiss-Prot :

P49841

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv

Species Reactivity Full Name :

Human Mouse Rat Bovine

Antibody Dilution :

WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.4

Concentration :

1.3 mg/ml

Specificity :

Detects ~47kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-169D, RRID: AB_2703785

Certificate of Analysis :

1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus Cell Membrane

Scientific Background :

Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer's, cancer and bipolar (4, 5).

References :

1. Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595. 2. Wang H. (2011) Cytokind. 53(2): 130-140. 3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47. 4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063. 5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-169_GSK3beta_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-169_GSK3-Beta_Antibody_WB_Human_Cell-line-lysates_1.png SPC-169_GSK3beta_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-169) Rabbit Anti-GSK3 Beta Antibody used in Western blot (WB) on Human Cell line lysates (SPC-169) Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-169)

more info or order :

StressMarq Biosciences product webpage