antibody

Oct-4 Antibody

600-401-A95S

25 µL

99.00 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunoprecipitation

Anti-Oct-4 (RABBIT) Antibody - 600-401-A95S

Oct-4 Antibody

This affinity purified antibody has been tested for use in ELISA and western blotting.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

0.59 mg/mL

by UV absorbance at 280 nm

(None)

25 µL

ul

1:350,000

User Optimized

User Optimized

1:1,000 - 1:1,500

Expiration date is three (3) months from date of opening.

IgG

POU5F1

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.

Dry Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested.  A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.

human, mouse, monkey, horse

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

rabbit anti-OCT4 antibody, OCT-4, OCT 4, Octamer binding protein 4 antibody, Octamer binding transcription factor 3 antibody, OTF3 antibody, OCT3, POU5F1, POU class 5 homeobox 1 antibody, POU domain class 5 transcription factor 1 antibody

Human

Oct-4, also known as POU domain transcription factor, Octomer- binding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression; the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation.

Prusa,A-R, Marton,E, Rosner,M, Bernaschek,G, and Hengstschläger,M. (2003) Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? Human Reproduction 18 (7), 1489-1493 Cauffman,G., Van de Velde,H., Liebaers,I., and Van Steirteghem,A. (2005) Oct-4 mRNA and protein expression during human preimplantation development Molecular Human Reproduction 11(3),173-181 Donovan, P.J. (2001) High Oct-ane fuel powers the stem cell. Nature Genet., 29, 246–247 Pesce, M. and Schöler, H.R. (2001) Oct-4: gatekeeper in the beginnings of mammalian development. Stem Cells, 19 (4), 271–278. Lin,L., Czerwiec,E., Keefe,D.L. (2004) Effect of ploidy and parental genome composition on expression of Oct-4 protein in mouse embryos. Gene Expression Patterns 4 (4), 433-441

Peptide

No

Yes

ELISA,IF Microscopy,Immunoprecipitation,Western Blot,

Primary Antibodies

Unconjugated

99.00 USD

Q01860

116235491

Anti-Oct4 Antibody - Western Blot