antibody

Protein Kinase C Beta Antibody

600-401-A76S

25 µL

99.00 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA

Anti-Protein Kinase C beta (RABBIT) Antibody - 600-401-A76S

Protein Kinase C Beta Antibody

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract.  Lysates from U251or BxPC3 cells are suggested for western blotting.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Polyclonal

1.0 mg/mL

by UV absorbance at 280 nm

(None)

25 µL

ul

1:75,000 - 1:80,000

1:1,000 - 1:2,000

Expiration date is three (3) months from date of opening.

IgG

PRKCB

Rabbit

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.

Dry Ice

Liquid (sterile filtered)

0.01% (w/v) Sodium Azide

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PKC beta protein.  A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence.  Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources.  Cross-reactivity with PKC beta from other sources has not been determined.

human, monkey

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

rabbit anti-Protein kinase C beta antibody, PKC ß, Protein kinase C beta type, PRKCB, PKCB, PRKCB1, PKC-beta, PKC-B

Human

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis.  PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption.  Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Graff JR, McNulty AM, Hanna KR, Konicek BW, Lynch RL, Bailey SN, Banks C, Capen A, Goode R, Lewis JE, Sams L, Huss KL, Campbell RM, Iversen PW, Neubauer BL, Brown TJ, Musib L, Geeganage S, Thornton D.  (2005) The protein kinase Cbeta-selective inhibitor, Enzastaurin (LY317615.HCl), suppresses signaling through the AKT pathway, induces apoptosis, and suppresses growth of human colon cancer and glioblastoma xenografts.  Cancer Res. 65(16):7462-9. Koivunen J, Aaltonen V, Koskela S, Lehenkari P, Laato M, Peltonen J. (2004) Protein kinase C alpha/beta inhibitor Go6976 promotes formation of cell junctions and inhibits invasion of urinary bladder carcinoma cells.  Cancer Res. 64(16):5693-701. Zhang J, Wang L, Petrin J, Bishop WR, Bond RW.(1993)  Characterization of site-specific mutants altered at protein kinase C beta 1 isozyme autophosphorylation sites. Proc Natl Acad Sci U S A. 90(13):6130-4. Flint AJ, Paladini RD, Koshland DE Jr. (1990) Autophosphorylation of protein kinase C at three separated regions of its primary sequence. Science. 249(4967):408-11.

Peptide

No

Yes

ELISA,Western Blot,

User Optimized

Primary Antibodies

Unconjugated

99.00 USD

P05771

47157322

Anti-PKC Beta Antibody - Western Blot