Histone H2AvD pS137 Antibody | Rockland Immunochemicals 600-401-914S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Histone H2AvD pS137 Antibody

catalog :

600-401-914S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

fruit fly

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

600-401-914S

Name :

Anti-Histone H2AvD pS137 (RABBIT) Antibody - 600-401-914S

Display Name :

Histone H2AvD pS137 Antibody

Application Note :

Histone H2AvD pS137 Antibody has been tested for use in ELISA, Immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:4,000 - 1:20,000

Immunohistochemistry Dilution :

2 ug/ml

Western Blot Dilution :

1:500 - 1:2,000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

HIS2AV

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Histone H2AvD pS137 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.

Species Reactivity :

Drosophila melanogaster

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-H2AvD pS137 antibody, rabbit anti-Histone H2A.v pS137 antibody, H2AvD protein antibody, H2A.F/Z, H2A.Z, H2AvD, His2AvD, His2Av

Target Species :

Drosophila melanogaster

Background :

Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX ''knock out'' mice have an increased incidence of cancer.

General Reference :

Mason,J.M., Ransom,J. and Konev,A.Y. (2004) A deficiency screen for dominant suppressors of telomeric silencing in Drosophila. Genetics 168 (3), 1353-1370. Kimura,K., Kodama,A., Hayasaka,Y. and Ohta,T. (2004) Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster. Development 131 (7), 1597-1606. Madigan, J.P., Chotkowski, H.L. and Glaser, R.L. (2002) DNA double strand breaks-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis. Nucleic Acid Res. 30:17; 368-3705.

Specific Reference :

Bosveld, F., Rana, A., van der Wouden, P. E., Lemstra, W., Ritsema, M., Kampinga, H. H., & Sibon, O. C. (2008). De novo CoA biosynthesis is required to maintain DNA integrity during development of the Drosophila nervous system. Human molecular genetics, 17(13), 2058-2069. Ryu T, Spatola B, Delabaere L, Bowlin K, Hopp H, Kunitake R, Karpen GH, Chiolo I. (2015) Heterochromatic breaks move to the nuclear periphery to continue recombinational repair. Nat Cell Biol. 2015 Nov;17(11):1401-11. doi: 10.1038/ncb3258. Epub 2015 Oct 26. Vujatovic O, Zaragoza K, Vaquero A, Reina O, Bernués J, Azorín F. (2012) Drosophila melanogaster linker histone dH1 is required for transposon silencing and to preserve genome integrity. Nucleic Acids Res. 2012 Jul;40(12):5402-14. doi: 10.1093/nar/gks224. Epub 2012 Mar 9.

Immunogen Type :

Peptide

Low Endotoxin :

Post Translational Modification :

Phosphorylation

Sample Size :

Yes

Target Domain of Reactivity :

Internal

Application Text :

ELISA,Immunohistochemistry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P08985

NCBI :

17738227

Primary Image Name :

Anti-Histone H2AvD pS137 Antibody - Western Blot