Beta Amyloid Antibody | Rockland Immunochemicals 600-401-253S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Beta Amyloid Antibody

catalog :

600-401-253S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

600-401-253S

Name :

Anti-Beta Amyloid (RABBIT) Antibody - 600-401-253S

Display Name :

Beta Amyloid Antibody

Application Note :

Affinity purified anti-beta amyloid detects beta amyloid in ELISA, IHC, WB, and IF. A 40-50 kD band consistent with a higher MW precursor is detected in western blot using whole tissue extracts from mouse brain. In general, we recommend the use of 4% PFA for paraffin embedded tissues and 10% formalin for frozen tissue for fixation.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

0.93mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000 - 1:50,000

Immunohistochemistry Dilution :

1:50-1:200

IF Microscopy Dilution :

1:50-1:200

Western Blot Dilution :

1:1000-1:5000

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

APP

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This affinity-purified antibody is directed against the amino terminal end of beta amyloid and is useful in determining its presence in various assays. Polyclonal anti-beta amyloid detects human and mouse beta amyloid. Cross reactivity with beta amyloid from other species is likely but has not been determined.

Species Reactivity :

human, mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-Beta Amyloid Antibody, ß-amyloid, Amyloid beta A4 protein, Alzheimer disease amyloid protein, Beta amyloid, A-beta, ABPP, APPI, Beta-amyloid precursor protein

Target Species :

Human

Background :

Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase.

General Reference :

N-terminal EFRH sequence of Alzheimer's beta-amyloid peptide represents the epitope of its anti-aggregating antibodies. (Frenkel D; J Neuroimmunol, 1998 Aug 1) Activation of nuclear factor-kappa B by beta-amyloid peptides and interferon-gamma in murine microglia. (Bonaiuto C; J Neuroimmunol, 1997 Jul) (Luo Y; Neurosci Lett, 1996 Oct 18) Alzheimer's beta-amyloid peptides induce inflammatory cascade in human vascular cells: the roles of cytokines and CD40. (Suo Z; Brain Res, 1998 Oct 5)

Immunogen Type :

Peptide

Low Endotoxin :

Sample Size :

Yes

Target Domain of Reactivity :

N-Terminus

Application Text :

ELISA,Immunohistochemistry,IF Microscopy,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P05067

NCBI :

NP_000475.1

Primary Image Name :

Anti-Beta Amyloid Antibody - Immunohistochemistry