Mouse IL-27/P28 Antibody | Rockland Immunochemicals 210-501-B54S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

Mouse IL-27/P28 Antibody

catalog :

210-501-B54S

quantity :

25 µL

price :

99.00 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3H12.F10

reactivity :

mouse

application :

western blot, ELISA, flow cytometry

product information

Catalog Number :

210-501-B54S

Name :

Anti-Mouse IL-27/p28 (RAT) Monoclonal Antibody - 210-501-B54S

Display Name :

Mouse IL-27/P28 Antibody

Application Note :

IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26,411 Da in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

3H12.F10

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:10,000

Flow Cytometry Dilution :

1 ug/mL

Western Blot Dilution :

1:1000

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG2a

Gene Name :

IL27

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

Anti-IL-27 / p28 antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse. Cross-reactivity with IL-27 from other sources has not been determined.

Species Reactivity :

Mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Subclass :

IgG2a kappa

Synonyms :

rat anti-IL-27/p28 antibody, rat anti-interleukin 27a antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

Target Species :

Mouse

Background :

Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.

General Reference :

Murugaiyan,G., Mittal,A. and Weiner,H.L. (2010) Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation. Proc. Natl. Acad. Sci. U.S.A. 107 (25), 11495-11500 PUBMED 20534530 Molle,C., Goldman,M. and Goriely,S. (2010) Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process. J. Immunol. 184 (4), 1784-1792 2008366

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Flow Cytometry,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

Q8K3I6

NCBI :

NP_663611.1

Primary Image Name :

Rockland anti IL-27/p28 rat monoclonal antibody-Flow Cytometry