MIP-3α Antibody | Rockland Immunochemicals 210-501-B51S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

MIP-3α Antibody

catalog :

210-501-B51S

quantity :

25 µL

price :

99.00 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

4N5F7

reactivity :

mouse

application :

western blot

product information

Catalog Number :

210-501-B51S

Name :

Anti-Mouse MIP-3α (RAT) Monoclonal Antibody - 210-501-B51S

Display Name :

MIP-3α Antibody

Application Note :

MIP-3α antibody has been tested for use in ELISA, IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Monoclonal

Clone ID :

4N5F7

Concentration Value :

1.0 mg/mL

Conjugation :

(None)

Size :

25 µL

Default Unit :

Western Blot Dilution :

0.5µg/mL

Expiration :

Expiration date is one (1) year from date of opening.

Format :

IgG

Gene Name :

CCL20

Host Animal :

Rat

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

Anti-MIP-3α (RAT) Monoclonal Antibody was produced in rat by repeated immunizations with mature full length recombinant mouse MIP-3α produced in E.coli followed by hybridoma development.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse MIP-3α protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3α from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3α from other sources has not been determined.

Species Reactivity :

mouse

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rat anti-MIP-3α antibody, CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Target Species :

Mouse

Background :

MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

General Reference :

Lisignoli,G., Manferdini,C., Codeluppi,K., Piacentini,A., Grassi,F., Cattini,L., Filardo,G. and Facchini,A. (2009) CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups. J. Cell. Physiol. 221 (1), 154-160. Punj,V., Matta,H., Schamus,S., Yang,T., Chang,Y. and Chaudhary,P.M. (2009) Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation. Blood 113 (22), 5660-5668. Reboldi,A., Coisne,C., Baumjohann,D., Benvenuto,F., Bottinelli,D., Lira,S., Uccelli,A., Lanzavecchia,A., Engelhardt,B. and Sallusto,F. (2009) C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat. Immunol. 10 (5), 514-523.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

O89093

NCBI :

NP_001153210.1

Primary Image Name :

Western Blot - MIP 3α Antibody