OspA Antibody | Rockland Immunochemicals 200-401-C13S product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

OspA Antibody

catalog :

200-401-C13S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Borrelia burgdorferi B31

application :

western blot, ELISA

product information

Catalog Number :

200-401-C13S

Name :

Anti-OspA (RABBIT) Antibody - 200-401-C13S

Display Name :

OspA Antibody

Application Note :

This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 28.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

>1:5,000

Western Blot Dilution :

1:1,000

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG

Gene Name :

ospA, BB_A15

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

MBP-fusion protein corresponding to Borrelia burgdorferi OspA protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspA protein. A BLAST analysis was used to suggest cross-reactivity with OspA from B. burgdorferi and sources based on 100% homology with the immunizing sequence. Cross-reactivity with OspA or Osp from other sources has not been determined.

Species Reactivity :

Borrelia burgdorferi

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-OspA Antibody, Outer surface protein A, Borrelia burgdorferi OspA

Target Species :

Borrelia burgdorferi

Background :

Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression.

General Reference :

Norris SJ. The dynamic proteome of Lyme disease Borrelia. (2006) Genome Biol. 7(3):209. Epub 2006 Mar 17. Ding W., Huang X., Yang X., Dunn J.J., Luft B.J., Koide S., Lawson C.L. (2000) Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J. Mol. Biol. 302:1153-1164(2000) [PubMed: 11183781] [Abstract] Stevenson B, Tilly K, and Rosa P.A. (1996) A Family of Genes Located on Four Separate 32-Kilobase Circular Plasmids in Borrelia burgdorferi B31. J Bacteriol 178(12) June 1996 p3508-3516

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

P0C926

NCBI :

NP_045688.1

Primary Image Name :

Anti-OspA Antibody - Western Blot