CCL2 Antibody | Rockland Immunochemicals 200-401-B60S product information

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product summary

company name :

Rockland Immunochemicals

product type :

antibody

product name :

CCL2 Antibody

catalog :

200-401-B60S

quantity :

25 µL

price :

99.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

cow

application :

western blot, ELISA

product information

Catalog Number :

200-401-B60S

Name :

Anti-Bovine CCL2 (RABBIT) Antibody - 200-401-B60S

Display Name :

CCL2 Antibody

Application Note :

This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kDa in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.

Buffer :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality :

Polyclonal

Concentration Value :

1.0 mg/mL

Concentration Definition :

by UV absorbance at 280 nm

Conjugation :

(None)

Size :

25 µL

Default Unit :

ELISA Dilution :

1:4500

Western Blot Dilution :

1 µg/ml

Expiration :

Expiration date is three (3) months from date of opening.

Format :

IgG

Gene Name :

CCL2

Host Animal :

Rabbit

General Disclaimer Note :

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen :

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.

Packing Type :

Dry Ice

Physical State :

Liquid (sterile filtered)

Preservative :

0.01% (w/v) Sodium Azide

Purity and Specificity :

This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for bovine CCL2 protein. A BLAST analysis was used to suggest cross-reactivity with CCL2 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Sus scrofa 85.5% and Bos Taurus 81.1%. Cross-reactivity with CCL2 from other sources has not been determined.

Species Reactivity :

bovine

Storage :

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Synonyms :

rabbit anti-CCL2 antibody, CCL-2, CCL 2, Chemokine (C-C motif) ligand 2, monocyte chemotactic (or chemoattractant) protein-1 (MCP-1)

Target Species :

Bovine

Background :

CCL2 is a small cytokine belonging to the CC chemokine family that recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing a signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a total molecular weight of approximately 8.8 kDa. The cell surface receptors that bind CCL2 are CCR2 and CCR4. CCL2 is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kB (NFkB). In human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted) may act as an autocrine loop in human osteoclast differentiation. CCL2 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.

General Reference :

Liptak,A.R., Sullivan,B.T., Henkes,L.E., Wijayagunawardane,M.P., Miyamoto,A., Davis,J.S., Rueda,B.R. and Townson,D.H. (2005). Cooperative expression of monocyte chemoattractant protein 1 within the bovine corpus luteum: evidence of immune cell-endothelial cell interactions in a coculture system Biol. Reprod. 72 (5), 1169-1176 PMID 15659710 Xu LL, Warren MK, Rose WL, Gong W, Wang JM (01 September 1996). Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro. J. Leukoc. Biol. 60 (3): 365–71. Craig MJ, Loberg RD (December 2006). CCL2 (Monocyte Chemoattractant Protein-1) in cancer bone metastases. Cancer Metastasis Rev. 25 (4): 611–9. doi:10.1007/s10555-006-9027-x. PMID 17160712.

Immunogen Type :

Recombinant Protein

Low Endotoxin :

Sample Size :

Yes

Application Text :

ELISA,Western Blot,

Other :

User Optimized

Category :

Primary Antibodies

Conjugation Name :

Unconjugated

2019 Price :

99.00 USD

UniProt :

A5PJF7

NCBI :

27806711

Primary Image Name :

Anti-Bovine CCL2 Antibody - Western Blot